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Combining metabolic evolution and systematic fed-batch optimization for efficient single-cell oil production from sugarcane bagasse

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  • Unrean, Pornkamol
  • Khajeeram, Sutamat
  • Champreda, Verawat

Abstract

We developed in this study an efficient production process of SCO from sugarcane bagasse hydrolysates using Y. lipolytica. We described a dynamic flux balance model strategy based on a genome-scale metabolic model of Y. lipolytica and SCO fermentation kinetics that applied for rational design of optimized fed-batch process with high single cell oil accumulation. Furthermore, metabolic evolution approach with buoyancy screening was implemented for selecting a high lipid producing yeast strain. The efficient conversion process combined model-based fed-batch process design and evolution-based strain optimization enabling high SCO titer of 45 g/L and 0.25 g-SCO/g-glucose yield from hydrolysates, a 6-fold improvement in titer and 1.4-fold improvement in yield over batch wild-type process. Through this combinatorial optimization effort, we demonstrated the cost-effective, lignocellulose-based SCO process that could meet techno-economic feasibility providing a sustainable alternative to vegetative oils for biofuels and oleochemicals synthesis.

Suggested Citation

  • Unrean, Pornkamol & Khajeeram, Sutamat & Champreda, Verawat, 2017. "Combining metabolic evolution and systematic fed-batch optimization for efficient single-cell oil production from sugarcane bagasse," Renewable Energy, Elsevier, vol. 111(C), pages 295-306.
    • Handle: RePEc:eee:renene:v:111:y:2017:i:c:p:295-306
    DOI: 10.1016/j.renene.2017.04.018
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    Cited by:

    1. Wang, Xuemin & Wang, Yanan & He, Qiaoning & Liu, Yantao & Zhao, Man & Liu, Yi & Zhou, Wenting & Gong, Zhiwei, 2022. "Highly efficient fed-batch modes for enzymatic hydrolysis and microbial lipogenesis from alkaline organosolv pretreated corn stover for biodiesel production," Renewable Energy, Elsevier, vol. 197(C), pages 1133-1143.
    2. Bao, Wenjun & Li, Zifu & Wang, Xuemei & Gao, Ruiling & Zhou, Xiaoqin & Cheng, Shikun & Men, Yu & Zheng, Lei, 2021. "Approaches to improve the lipid synthesis of oleaginous yeast Yarrowia lipolytica: A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 149(C).

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