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Cell aggregation in monolayer culture: Clues to a universal kinetics

Author

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  • da Silva, P.C.A.
  • Santos, A.A.
  • Alves, S.G.
  • Martins, M.L.

Abstract

Aggregation of animal cells in culture comprises a series of motility, collision and adhesion processes of basic relevance for tissue engineering, bioseparations, oncology research and in vitro drug testing. In the present paper, we characterize the aggregation kinetics of normal and cancer cells in culture by determining their cluster size distribution functions. Our experiments reveal that the complementary cumulative distributions for eight cell lines from epithelial and mesenchymal origins are described by stretched exponential functions. Also, we find that as time evolves and the cell density increases, a dynamical phase transition occurs from an aggregation regime described by monotonically decreasing cluster size distributions to another one in which these distributions are nonmonotonic, exhibiting local maxima at large cluster sizes. Subtly, the universality of the stretched exponential distributions admits distinct cluster growth processes controlled by motility and adhesion traits of the cells.

Suggested Citation

  • da Silva, P.C.A. & Santos, A.A. & Alves, S.G. & Martins, M.L., 2018. "Cell aggregation in monolayer culture: Clues to a universal kinetics," Physica A: Statistical Mechanics and its Applications, Elsevier, vol. 510(C), pages 725-740.
    • Handle: RePEc:eee:phsmap:v:510:y:2018:i:c:p:725-740
    DOI: 10.1016/j.physa.2018.07.035
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    References listed on IDEAS

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    1. Sean H J Kim & Wei Yu & Keith Mostov & Michael A Matthay & C Anthony Hunt, 2009. "A Computational Approach to Understand In Vitro Alveolar Morphogenesis," PLOS ONE, Public Library of Science, vol. 4(3), pages 1-17, March.
    2. Priscila C A da Silva & Tiago V Rosembach & Anésia A Santos & Márcio S Rocha & Marcelo L Martins, 2014. "Normal and Tumoral Melanocytes Exhibit q-Gaussian Random Search Patterns," PLOS ONE, Public Library of Science, vol. 9(9), pages 1-13, September.
    3. Mendes, Rosemairy L. & Santos, Anésia A. & Martins, M.L. & Vilela, M.J., 2001. "Cluster size distribution of cell aggregates in culture," Physica A: Statistical Mechanics and its Applications, Elsevier, vol. 298(3), pages 471-487.
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