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Detection of ganglioside clustering in DOPC bilayers by 1H-NMR spectroscopy

Author

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  • D’Emiliano, D.
  • Casieri, C.
  • Paci, M.
  • De Luca, F.

Abstract

1H-NMR spectroscopy has been used to investigate the formation of GM1 clusters in DOPC liposome bilayer. The 1H-DOPC NMR spectra show that the presence of GM1 modifies some resonance lines. These modifications are imputable to the interaction of DOPC with GM1. Above a certain GM1 concentration the trend of such distortions suggests the formation of GM1 aggregates. This work provides a new and simple NMR proof of the GM1 clustering in DOPC liposomes and therefore on model cell membranes; it allows to estimate the mean size of GM1 aggregates and the GM1 concentration at which clustering starts. In addition, the behaviour of the 1H-NMR line-width shows that the presence of GM1 modifies the bilayer arrangement making it more mobile, especially at GM1-clustering concentration.

Suggested Citation

  • D’Emiliano, D. & Casieri, C. & Paci, M. & De Luca, F., 2007. "Detection of ganglioside clustering in DOPC bilayers by 1H-NMR spectroscopy," Physica A: Statistical Mechanics and its Applications, Elsevier, vol. 374(1), pages 293-303.
    • Handle: RePEc:eee:phsmap:v:374:y:2007:i:1:p:293-303
    DOI: 10.1016/j.physa.2006.07.021
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    References listed on IDEAS

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    1. Kai Simons & Elina Ikonen, 1997. "Functional rafts in cell membranes," Nature, Nature, vol. 387(6633), pages 569-572, June.
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