Nematicidal Activity of Some Chemical and Bio – Control Agents Against 2ND Stage Juveniles of Wheat Seed GALL Nematode Anguina tritici

This study consisted of two in - vitro bioassay, in the 1st one four medicinal plants were selected to test nematicidal activity of their aqueous extracts (A.E) against 2nd stage juveniles (Js2) of wheat seed gall nematode Anguina tritici included: leaves of Mint, Datura, Eminium (Zilikeraba, Kary) and Pomegranate peels. Results revealed that the highest nematode mortality (92.9%) was recorded in the A.E. of Mint leaves, while the lowest (76.7%) in A.E. of Datura leaves regardless, their mixing ratio with distilled water (D.W.). Nematode mortality significantly increased by increasing mixing ratio. and in general the highest nematode mortality (100 %) was recorded by A.E. of Eminium leaves after a week of immersing J2 in its higher mixing ratio (8ml A.E.: 10 ml D.W.) with no significant difference compared to A.E. of mint leaves at its last two mixing ratio (4 ml A.E.: 10 ml D.W. and 8 ml A.E.:10 ml D.W.), whilst the lowest nematode mortality (66.1%) was recorded by the same plant (Eminium) at its lower mixing ratio (1ml A.E. : 10 ml D.W.). In the 2nd in – vitro bioassay Velum prime as nematicide, Albendazole as anthelmintic drug and BM Root Pan as bio- control agent were selected to test their nematicidal acivity against Js2 of A. tritici. Results showed that the highest nematode mortality (46.5%) was recorded by Velum prime with significant difference compared to the other control agents meanwhile, the lowest mortality (31.25%) was reported by BM-Root-Pan regardless their concentrations. In general the highest nematode mortality (78 %) was found when they were immersed in Velum prime at concentration 5ppm with significant difference compared to the other concentrations for the same nematicide and for the other control agents followed by Albendazole drug (62%.) at concentration 3mg whilst, the lowest nematode mortality was recorded by BM - Root – Pan at concentration 0.1x 10⁶.