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Immunohistological changes in skin wounds during the early periods of healing in a rat model

Author

Listed:
  • F. Sabol

    (Pavol Jozef Safarik University, Kosice, Slovak Republic
    East-Slovak Institute of Cardiovascular Diseases, Kosice, Slovak Republic)

  • L. Dancakova

    (University of Veterinary Medicine and Pharmacy, Kosice, Slovak Republic)

  • P. Gal

    (East-Slovak Institute of Cardiovascular Diseases, Kosice, Slovak Republic
    University of Veterinary Medicine and Pharmacy, Kosice, Slovak Republic
    Charles University, Prague, Czech Republic)

  • T. Vasilenko

    (University of Veterinary Medicine and Pharmacy, Kosice, Slovak Republic
    Slovak Health University, Kosice, Slovak Republic
    Kosice-Saca Hospital, Kosice, Slovak Republic)

  • M. Novotny

    (Pavol Jozef Safarik University, Kosice, Slovak Republic)

  • K. Smetana

    (Charles University, Prague, Czech Republic)

  • L. Lenhardt

    (University of Veterinary Medicine and Pharmacy, Kosice, Slovak Republic)

Abstract

The complexity of the wound healing process, which is still poorly understood, prompted us to perform an immunohistochemical investigation using rat skin as an in vivo model. Fifteen Sprague-Dawley rats were included in the experiment. Two round full thickness wounds, 4 mm in diameter, were made on the backs of all rats. Haematoxylin and eosin basic staining as well as antibodies against wide spectrum keratin, keratin 10, keratin 14, α-smooth muscle actin, vimentin, fibronectin, collagens Type 1 and 3, and the transcription factor Sox-2 were applied to paraffin and frozen sections of skin wound specimens two, six and fourteen days after surgery, respectively. New hair follicles with Sox-2-positive cells were present after fourteen days; keratin/vimentin positivity was restricted to specimens of day two. Collagen-3 expression prevailed over collagen-1 expression at all evaluated time intervals, except in the uninjured part of the dermis. In conclusion, rat skin wound healing is a dynamic process which can serve as a model for studying phenomena such as cell-cell interactions and transitions in vivo.

Suggested Citation

  • F. Sabol & L. Dancakova & P. Gal & T. Vasilenko & M. Novotny & K. Smetana & L. Lenhardt, 2012. "Immunohistological changes in skin wounds during the early periods of healing in a rat model," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 57(2), pages 77-82.
    • Handle: RePEc:caa:jnlvet:v:57:y:2012:i:2:id:5253-vetmed
    DOI: 10.17221/5253-VETMED
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    References listed on IDEAS

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    1. I. Yaman & A.S. Durmus & S. Ceribasi & M. Yaman, 2010. "Effects of Nigella sativa and silver sulfadiazine on burn wound healing in rats," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 55(12), pages 619-624.
    2. P. Gal & R. Kilik & M. Mokry & B. Vidinsky & T. Vasilenko & S. Mozes & N. Bobrov & Z. Tomori & J. Bober & L. Lenhardt, 2008. "Simple method of open skin wound healing model in corticosteroid-treated and diabetic rats: standardization of semi-quantitative and quantitative histological assessments," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 53(12), pages 652-659.
    3. C. Hu & Y. Ding & J. Chen & D. Liu & M. Ding & Y. Zhang, 2009. "Treatment of corneal epithelial wounds in dogs using basic fibroblast growth factor," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 54(6), pages 280-286.
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    Cited by:

    1. A.S. Durmus & M. Yaman & H.N. Can, 2012. "Effects of extractum cepae, heparin, allantoin gel and silver sulfadiazine on burn wound healing: an experimental study in a rat model," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 57(6), pages 287-292.

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    1. A.S. Durmus & M. Yaman & H.N. Can, 2012. "Effects of extractum cepae, heparin, allantoin gel and silver sulfadiazine on burn wound healing: an experimental study in a rat model," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 57(6), pages 287-292.

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