IDEAS home Printed from https://ideas.repec.org/a/caa/jnlvet/v50y2005i7id5627-vetmed.html
   My bibliography  Save this article

Production and characterisation of monoclonal antibodies for the detection of AOZ, a tissue bound metabolite of furazolidone

Author

Listed:
  • M. Vass

    (Veterinary Research Institute, Brno, Czech Republic)

  • L. Kotkova

    (Veterinary Research Institute, Brno, Czech Republic)

  • I. Diblikova

    (Veterinary Research Institute, Brno, Czech Republic)

  • Z. Nevorankova

    (Veterinary Research Institute, Brno, Czech Republic)

  • K.M. Cooper

    (Queen's University Belfast, Department of Veterinary Science, Northern Ireland, UK)

  • D.G. Kennedy

    (Chemical Surveillance Branch, Veterinary Sciences Division, Department of Agriculture & Rural Development, Belfast, Northern Ireland, UK)

  • M. Franek

    (Veterinary Research Institute, Brno, Czech Republic)

Abstract

3-amino-2-oxazolidinone (AOZ) is a tissue bound toxic metabolite derived from the nitrofuran antibiotic, furazolidone. AOZ is detected in the derivatised form of 3-{[(2-nitrophenyl) methylene] amino}-2-oxa-zolidinone (NP AOZ). 3-{[(3-carboxyphenyl)-methylene] amino-2-oxazolidinone (CP AOZ) was used as the immunising hapten for the production of monoclonal antibodies against NP AOZ. Monoclonal antibodies were produced using hybridomas from the fusion of murine myeloma cells and spleen cells isolated from BALB/c mice immunised with CP AOZ-ethylenediamine-human serum albumin (CP AOZ-ed-HSA). The antibody production in ascitic fluids from clones 3B8/2B9 and 2D11/A4 was monitored during a 16 month period. Repeated cultures of these hybridomas, followed by injection into mice and cloning did not change the assay parameters. Clone 2D11/A4 exhibited long term stability in antibody production throughout the experiment whereas clone 3B8/2B9 demonstrated variability in particular antibody yields whilst retaining assay sensitivity. Reasons for this production variability in clones are discussed. In an optimised direct ELISA format, the antibodies exhibited a 50% binding inhibition in the range of 0.52-1.15 ng/ml with NP AOZ (0.22 -0.50 ng/ml, respective AOZ equivalents) and showed high specificity towards this analyte. The sensitivity of monoclonal antibodies incorporated into the ELISA is compatible with the European Union MRLP and is currently in use for routine analysis.

Suggested Citation

  • M. Vass & L. Kotkova & I. Diblikova & Z. Nevorankova & K.M. Cooper & D.G. Kennedy & M. Franek, 2005. "Production and characterisation of monoclonal antibodies for the detection of AOZ, a tissue bound metabolite of furazolidone," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 50(7), pages 300-310.
  • Handle: RePEc:caa:jnlvet:v:50:y:2005:i:7:id:5627-vetmed
    DOI: 10.17221/5627-VETMED
    as

    Download full text from publisher

    File URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5627-VETMED.html
    Download Restriction: free of charge

    File URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5627-VETMED.pdf
    Download Restriction: free of charge

    File URL: https://libkey.io/10.17221/5627-VETMED?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. M. Vass & K. Hruska & M. Franek, 2008. "Nitrofuran antibiotics: a review on the application, prohibition and residual analysis," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 53(9), pages 469-500.
    2. M. Franek & I. Diblikova & M. Vass & L. Kotkova & K. Stastny & K. Frgalova & K. Hruska, 2006. "Validation of a monoclonal antibody-based ELISA for the quantification of the furazolidone metabolite (AOZ) in eggs using various sample preparation," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 51(5), pages 248-257.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlvet:v:50:y:2005:i:7:id:5627-vetmed. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.