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Infrared spectroscopy-based metabolomic analysis of maize growing under different nitrogen nutrition

Author

Listed:
  • M. Pavlík

    (Isotope Laboratory, Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech Republic)

  • D. Pavlíková

    (Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic)

  • S. Vašíčková

    (Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic)

Abstract

For metabolomic analysis of maize plants growing under different nitrogen nutrition sequential extraction of fresh biomass was used and isolated fractions were characterized and evaluated using IR spectra. The IR spectra of individual fractions were evaluated in relation to the nitrogen rates (2 g or 4 g N), to applied fertilizers (ammonium nitrogen or urea ammonium nitrate solution) and sampling period. For butanol fraction, typical bands of flavonoids, polar phospholipids, steryl glycosides, analogues of ecdysteroids were characterized. The IR spectra of BuOH fraction showed changes of relative contents of isolated compounds mainly affected by nitrogen rates. For water fraction bands of organic acids, salts of organic acids, flavonoid glycosides and oligopeptides (phytochelatins and/or glutathione) were the most significant. The results showed an increased induction of oxalic acid in plants after 4 g N application. Degradation of this acid induced oxidative stress, therefore strong correlation among contents of oxalic acid, flavonoids and compounds with amide nitrogen (glutathione) was observed in plants growing under 4 g nitrogen nutrition. The glutathione-ascorbate cycle protects plants against oxidative damage.

Suggested Citation

  • M. Pavlík & D. Pavlíková & S. Vašíčková, 2010. "Infrared spectroscopy-based metabolomic analysis of maize growing under different nitrogen nutrition," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 56(11), pages 533-540.
    • Handle: RePEc:caa:jnlpse:v:56:y:2010:i:11:id:190-2010-pse
    DOI: 10.17221/190/2010-PSE
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    References listed on IDEAS

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    1. O. Kozlovský & J. Balík & J. Černý & M. Kulhánek & M. Kos & M. Prášilová, 2009. "Influence of nitrogen fertilizer injection (CULTAN) on yield, yield components formation and quality of winter wheat grain," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 55(12), pages 536-543.
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