IDEAS home Printed from https://ideas.repec.org/a/caa/jnlpse/v53y2007i11id2307-pse.html
   My bibliography  Save this article

The effect of brassinosteroids on radish (Raphanus sativus L.) seedlings growing under cadmium stress

Author

Listed:
  • S. Anuradha

    (Department of Botany, Osmania University, Hyderabad, India)

  • S.S.R. Rao

    (Department of Botany, Osmania University, Hyderabad, India)

Abstract

The effect of 24-epibrassinolide and 28-homobrassinolide on seed germination and seedling growth of radish (Raphanus sativus L.) was studied under cadmium toxicity. The impact of brassinosteroids (BRs) on free proline levels and the activity of antioxidant enzymes catalase (CAT; EC 1.11.1.6), peroxidase (POD; EC 1.11.1.7), superoxide dismutase (SOD; EC 1.15.1.1), ascorbic peroxidase (APOX; EC 1.11.1.11) and guaiacol peroxidase (GPX; EC 1.11.1.7) in radish seedlings under Cd toxicity was evaluated. The effect of BRs on the activity of ascorbic acid oxidase (AAO; EC 1.10.3.3) and lipid peroxidation in radish seedlings challenged with Cd stress was also investigated. BRs supplementation alleviated the toxic effect of the heavy metal and increased the percentage of seed germination and seedling growth. Out of the two substances, HBL was found to be more effective than EBL in stress alleviation. HBL (3µM) alleviated the toxic effect of the heavy metal and increased the percentage of seed germination by 57% over Cd and 20% over unstressed control. Similarly supplementation of HBL (3µM) caused an increase of 156%, 78% and 91% in length, fresh weight and dry weight of seedling, respectively, over Cd treatment alone. The amelioration of seedling growth by BRs under metal toxicity was associated with enhanced levels of free proline. The activities of antioxidant enzymes CAT, SOD, APOX and GPX were increased in the seedlings from treatments with Cd along with BRs. Brassinosteroid treatment reduced the activity of POD and AAO in heavy metal stressed seedlings. Lipid peroxidation induced by Cd was found reduced with the supplementation of BRs. The results obtained in the study clearly indicated the ameliorative influence of brassinosteroids on the inhibitory effect of Cd toxicity.

Suggested Citation

  • S. Anuradha & S.S.R. Rao, 2007. "The effect of brassinosteroids on radish (Raphanus sativus L.) seedlings growing under cadmium stress," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 53(11), pages 465-472.
    • Handle: RePEc:caa:jnlpse:v:53:y:2007:i:11:id:2307-pse
    DOI: 10.17221/2307-PSE
    as

    Download full text from publisher

    File URL: http://pse.agriculturejournals.cz/doi/10.17221/2307-PSE.html
    Download Restriction: free of charge
    File URL: http://pse.agriculturejournals.cz/doi/10.17221/2307-PSE.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/2307-PSE?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. P. Procházka & P. Štranc & I. Kupka & J. Štranc & K. Pazderů, 2015. "Forest seed treatment with brassinosteroids to increase their germination under stress conditions," Journal of Forest Science, Czech Academy of Agricultural Sciences, vol. 61(7), pages 291-296.
    2. M. Kroutil & A. Hejtmánková & J. Lachman, 2010. "Effect of spring wheat (Triticum aestivum L.) treatment with brassinosteroids on the content of cadmium and lead in plant aerial biomass and grain," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 56(1), pages 43-50.
    3. A. Temel & N. Gozukirmizi, 2012. "Effects of homobrassinolide in barley callus culture," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 58(10), pages 441-445.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlpse:v:53:y:2007:i:11:id:2307-pse. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.