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In vitro regeneration of Pistacia vera L. from nodal explants

Author

Listed:
  • B. Benmahioul

    (Department of Forest Resources, Faculty of Natural Sciences and Life, Earth Sciences and the Universe, University of Abou-Bekr Belkaïd Tlemcen, Tlemcen, Algeria)

  • M. Kaïd-Harche

    (Department of Biotechnology, Faculty of Sciences, University of Sciences and Technology of Oran Mohamed Boudiaf, Oran, Algeria)

  • F. Daguin

    (Research Institute of Horticulture and Seeds, University of Angers, Angers, France)

Abstract

To enhance the induction of shoots, the excised nodes were cultured on Murashige and Skoog medium containing different cytokinins and various concentrations. The best conditions for shoot induction and growth were with 6-benzyladenine at 1.0 mg.l-1. For axillary shoot proliferation, the excellent result was obtained using 2.0 mg.l-1 meta-topoline. Well-developed shoots (more than 2 cm in length) were successfully rooted ex vitro at 82% by treatment with commercial rooting powder (2% indole-3-butyric acid; Rhizopon®). Rooted plantlets were acclimatized in the greenhouse and the survival rate of transplantation reached 80%.

Suggested Citation

  • B. Benmahioul & M. Kaïd-Harche & F. Daguin, 2016. "In vitro regeneration of Pistacia vera L. from nodal explants," Journal of Forest Science, Czech Academy of Agricultural Sciences, vol. 62(5), pages 198-203.
    • Handle: RePEc:caa:jnljfs:v:62:y:2016:i:5:id:82-2015-jfs
    DOI: 10.17221/82/2015-JFS
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    References listed on IDEAS

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    1. B. Benmahioul & F. Daguin & M. Kaïd-Harche, 2015. "Cryopreservation of Pistacia vera embryonic axes," Journal of Forest Science, Czech Academy of Agricultural Sciences, vol. 61(4), pages 182-187.
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