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Determination of in vitro antibacterial activity of plant oils containing medium-chain fatty acids against Gram-positive pathogenic and gut commensal bacteria

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  • Petra Hovorková

    (Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic)

  • Klára Laloučková

    (Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic
    Department of Physiology of Nutrition and Animal Product Quality, Institute of Animal Science, Prague-Uhříněves, Czech Republic)

  • Eva Skřivanová

    (Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic
    Department of Physiology of Nutrition and Animal Product Quality, Institute of Animal Science, Prague-Uhříněves, Czech Republic)

Abstract

Increasing antibiotic resistance has led to a ban on antibiotic use in feed additives in the EU. Therefore, new non-antibiotic, pathogen-inhibiting agents are urgently needed. Inhibitory effects of eight plant oils containing medium-chain fatty acids (MCFAs) were evaluated against Gram-positive pathogenic and beneficial bacteria. The oils tested were palm, red palm, palm kernel (Elaeis guineensis), coconut (Cocos nucifera), babassu (Attalea speciosa), murumuru (Astrocaryum murumuru), tucuma (Astrocaryum vulgare), and Cuphea oil (Cuphea ignea); the method used was broth microdilution, and the findings were expressed as minimum inhibitory concentration (80%). Both hydrolyzed and unhydrolyzed forms of the oils were tested. MCFA hydrolysis was catalyzed by porcine pancreas lipase. The selective effect of the hydrolyzed forms of tested oils was highly evident. While the hydrolyzed oils were active against all tested bacteria (Clostridium perfringens, Enterococcus cecorum, Listeria monocytogenes, and Staphylococcus aureus), at 0.14-4.5 mg/ml, the same oils did not show any effect on commensal bacteria (Bifidobacterium spp. and Lactobacillus spp.). Tucuma and Cuphea seed oils showed the strongest antibacterial activity. Unhydrolyzed forms of all tested oils exerted no antibacterial effect against any test bacteria. This study, thus, forms a basis for the development of selective inhibitors in animal husbandry.

Suggested Citation

  • Petra Hovorková & Klára Laloučková & Eva Skřivanová, 2018. "Determination of in vitro antibacterial activity of plant oils containing medium-chain fatty acids against Gram-positive pathogenic and gut commensal bacteria," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 63(3), pages 119-125.
    • Handle: RePEc:caa:jnlcjs:v:63:y:2018:i:3:id:70-2017-cjas
    DOI: 10.17221/70/2017-CJAS
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    References listed on IDEAS

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    1. Małgorzata Paulina Majewska & Renata Miltko & Grzegorz Bełżecki & Jacek Skomiał & Barbara Kowalik, 2017. "Supplementation of rapeseed and linseed oils to sheep rations: effects on ruminal fermentation characteristics and protozoal populations," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 62(12), pages 527-538.
    2. X.J. Dai & C. Wang & Q. Zhu, 2011. "Milk performance of dairy cows supplemented with rape seed oil, peanut oil, and sunflower seed oil," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 56(4), pages 181-191.
    3. K. Karásková & P. Suchý & E. Straková, 2015. "Current use of phytogenic feed additives in animal nutrition: a review," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 60(12), pages 521-530.
    4. E. Skřivanová & Š. Pražáková & O. Benada & P. Hovorková & M. Marounek, 2014. "Susceptibility of Escherichia coli and Clostridium perfringens to sucrose monoesters of capric and lauric acid," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 59(8), pages 374-380.
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    Cited by:

    1. Klára Laloučková & Lucie Malá & Paula Slaničková & Eva Skřivanová, 2019. "In vitro antimicrobial effect of palm oils rich in medium-chain fatty acids against mastitis-causing Gram-positive bacteria," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 64(8), pages 325-331.

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