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Effect of protein secondary structures in mixed feedstuff detected by Fourier transform infrared spectroscopy on ruminal protein degradation kinetics

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  • Dasen Liu

    (College of Animal Science and Technology, Northeast Agricultural University, Harbin, P.R. China
    College of Science, Northeast Agricultural University, Harbin, P.R. China)

  • Yanfang Li

    (College of Animal Science and Technology, Northeast Agricultural University, Harbin, P.R. China)

  • Guangliang Zhang

    (College of Animal Science and Technology, Northeast Agricultural University, Harbin, P.R. China)

  • Peng Zhang

    (College of Science, Northeast Agricultural University, Harbin, P.R. China)

  • Penghua Wu

    (College of Animal Science and Technology, Northeast Agricultural University, Harbin, P.R. China)

  • Shai Wang

    (College of Animal Science and Technology, Northeast Agricultural University, Harbin, P.R. China)

  • Xiuxiu Wang

    (College of Animal Science and Technology, Northeast Agricultural University, Harbin, P.R. China)

Abstract

The objective of this study was to investigate the relationship between the protein secondary structure and the protein rumen degradation kinetics and the protein fractions of mixed feedstuffs of soybean meal with distillers dried grains with solubles (DDGS) at five mixed ratios (DDGS to soybean meal: 100 : 0, 70 : 30, 50 : 50, 30 : 70, 0 : 100). The Fourier transform infrared (FTIR) as a novel and cheap approach was used to detect the protein secondary structure, and the in situ nylon bag method was used to measure degradation kinetics of protein. Protein fractions were classified based on the Cornell net carbohydrate protein system. The results showed that (1) with the increasing soybean meal rate, the ruminal degraded protein and fractions of PB1 and PB2 were changed, (2) a higher α-helix to β-sheet ratio could result in a higher ruminally degraded protein, lower PB3 and PC, and higher PB1 and PB2 fractions in the feedstuff. So, mixing processing changed the feedstuff protein molecular structure spectral feature, which could influence the rumen degradation kinetics and protein fractions. It was inferred that protein degradation rate in mixed feedstuff can be measured by FTIR.

Suggested Citation

  • Dasen Liu & Yanfang Li & Guangliang Zhang & Peng Zhang & Penghua Wu & Shai Wang & Xiuxiu Wang, 2017. "Effect of protein secondary structures in mixed feedstuff detected by Fourier transform infrared spectroscopy on ruminal protein degradation kinetics," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 62(3), pages 89-97.
    • Handle: RePEc:caa:jnlcjs:v:62:y:2017:i:3:id:94-2015-cjas
    DOI: 10.17221/94/2015-CJAS
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    References listed on IDEAS

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    1. D. Liu & P. Wu & P. Jiao, 2015. "Researching rumen degradation behaviour of protein by FTIR spectroscopy," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 60(1), pages 25-32.
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    Cited by:

    1. Petr Homolka & Marie Koukolová & Veronika Koukolová, 2018. "Organic matter and crude protein digestibility predicted from nitrogen and fibre fractionation of festulolium hybrids," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 63(7), pages 272-279.

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    1. Petr Homolka & Marie Koukolová & Veronika Koukolová, 2018. "Organic matter and crude protein digestibility predicted from nitrogen and fibre fractionation of festulolium hybrids," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 63(7), pages 272-279.

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