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Researching rumen degradation behaviour of protein by FTIR spectroscopy

Author

Listed:
  • D. Liu

    (College of Animal Science and Technology, Northeast Agricultural University, Harbin, P.R. China)

  • P. Wu

    (College of Animal Science and Technology, Northeast Agricultural University, Harbin, P.R. China)

  • P. Jiao

    (College of Animal Science and Technology, Northeast Agricultural University, Harbin, P.R. China)

Abstract

The objectives of the present study were: (1) to detect protein secondary structures using the Fourier transform infrared spectroscopy (FTIR), and (2) to investigate the relationship between protein secondary structures and their rumen degradation kinetics in dairy cattle. The study was carried out using FTIR to estimate protein molecular structure of eight different types of samples, such as soybean meal, cottonseed meal, rapeseed meal, distilled dried grains with solubles, and corn. Nutritive values of these samples were determined using the Cornell Net Carbohydrate and Protein System. The results showed that: (1) different feed protein sources had different percentages and ratios of α-helixes and β-sheets in their protein secondary structures, (2) the higher protein α-helix to β-sheet ratio resulted in lower PB3 and PC, and higher PB1 and PB2 in the feeds, and (3) the α-helix to β-sheet ratio of the rumen degradation residues was changed along with the rumen degradation.

Suggested Citation

  • D. Liu & P. Wu & P. Jiao, 2015. "Researching rumen degradation behaviour of protein by FTIR spectroscopy," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 60(1), pages 25-32.
    • Handle: RePEc:caa:jnlcjs:v:60:y:2015:i:1:id:7908-cjas
    DOI: 10.17221/7908-CJAS
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    Citations

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    Cited by:

    1. Petr Homolka & Marie Koukolová & Veronika Koukolová, 2018. "Organic matter and crude protein digestibility predicted from nitrogen and fibre fractionation of festulolium hybrids," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 63(7), pages 272-279.
    2. Dasen Liu & Yanfang Li & Guangliang Zhang & Peng Zhang & Penghua Wu & Shai Wang & Xiuxiu Wang, 2017. "Effect of protein secondary structures in mixed feedstuff detected by Fourier transform infrared spectroscopy on ruminal protein degradation kinetics," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 62(3), pages 89-97.

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