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Effect of low-density lipoprotein addition to soybean lecithin-based extenders on bull spermatozoa following freezing-thawing - preliminary results

Author

Listed:
  • O. Šimoník

    (Department of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic)

  • R. Rajmon

    (Department of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic)

  • L. Stádník

    (Department of Animal Husbandry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic)

  • J. Šichtař

    (Department of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic)

  • J. Beran

    (Department of Animal Husbandry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic)

  • J. Ducháček

    (Department of Animal Husbandry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic)

  • P. Hodek

    (Department of Biochemistry, Faculty of Science, Charles University in Prague, Prague, Czech Republic)

  • P. Trefil

    (BIOPHARM, Research Institute of Biopharmacy and Veterinary Drugs, Jílové u Prahy, Czech Republic)

Abstract

Soybean lecithin-based extenders represent an alternative to extenders containing egg yolk, but there has been discussion about their cryoprotective efficacy. Low-density lipoprotein (LDL) was proved as a cryoprotective agent, which can replace egg yolk in extenders. The aim of this study was to investigate the effects of LDL addition to the soybean extenders on their cryoprotective properties. The effect of the LDL of our production was verified using commercial egg yolk extender BULLXcell®, 6%, 8%, and 10% LDL (v/v) as an egg yolk replacement. The effects of LDL addition to the soybean lecithin-based extenders in concentrations of 4%, 6%, and 8% (v/v) were tested using extenders AndroMed® and Bioxcell®. In total, 64 samples from eight bulls were evaluated. Kinematic parameters of spermatozoa, resulting from Computer Assisted Sperm Analysis, and their viability, evaluated by fluorescent technique, were assessed immediately after thawing and after 2 hours. The quality of LDL compared to other studies was confirmed, and the beneficial effects of egg yolk replacement by LDL were proved in extender BULLXcell®. 8% LDL provided the best values for the majority of kinematic parameters (P < 0.05), without effect on total motility (P > 0.05). Furthermore, addition of 4%, 6%, and 8% LDL to the soybean lecithin-based extender Bioxcell® showed a positive effect on the majority of kinematic parameters of spermatozoa (P < 0.05) at both times of incubation. However there was no significant influence on total motility (P > 0.05). Viability was higher after thawing in the case of 8% LDL (P < 0.05). However, there was no consistent effect of LDL addition to the AndroMed® extender. In conclusion, cryoprotective properties of the semen extenders based on a soybean lecithin can be improved by the addition of LDL.

Suggested Citation

  • O. Šimoník & R. Rajmon & L. Stádník & J. Šichtař & J. Beran & J. Ducháček & P. Hodek & P. Trefil, 2016. "Effect of low-density lipoprotein addition to soybean lecithin-based extenders on bull spermatozoa following freezing-thawing - preliminary results," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 61(12), pages 560-567.
    • Handle: RePEc:caa:jnlcjs:v:61:y:2016:i:12:id:27-2016-cjas
    DOI: 10.17221/27/2016-CJAS
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    References listed on IDEAS

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    1. Z.A. Muchlisin & W.N. Nadiah & N. Nadiya & N. Fadli & A. Hendri & M. Khalil & M.N. Siti-Azizah, 2015. "Exploration of natural cryoprotectants for cryopreservation of African catfish, Clarias gariepinus, Burchell 1822 (Pisces: Clariidae) spermatozoa," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 60(1), pages 10-15.
    2. V. Dzyuba & J. Cosson & B. Dzyuba & M. Rodina, 2015. "Oxidative stress and motility in tench Tinca tinca spermatozoa," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 60(6), pages 250-262.
    3. M. Meamar & A.Z. Shahneh & M.J. Zamiri & S. Zeinoaldini & H. Kohram & M.R. Hashemi & S. Asghari, 2016. "Preservation effects of melatonin on the quality and fertility of native Fars rooster semen during liquid storage," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 61(1), pages 42-48.
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    Cited by:

    1. Adéla Krejcárková & Petra Folková & Ondřej Šimoník & Martina Šašková & Romana Krejčířová & Ondřej Drábek & Radko Rajmon, 2017. "Effects of zearalenone, α-zearalenol, and genistein on boar sperm motility in vitro," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 62(10), pages 435-445.

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