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Equilibration and freezing interactions affecting bull sperm characteristics after thawing

Author

Listed:
  • M. Doležalová

    (Czech University of Life Sciences Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Animal Husbandry, Prague, Czech Republic)

  • L. Stádník

    (Czech University of Life Sciences Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Animal Husbandry, Prague, Czech Republic)

  • Z. Biniová

    (Czech University of Life Sciences Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Animal Husbandry, Prague, Czech Republic)

  • J. Ducháček

    (Czech University of Life Sciences Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Animal Husbandry, Prague, Czech Republic)

  • R. Stupka

    (Czech University of Life Sciences Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Animal Husbandry, Prague, Czech Republic)

Abstract

The objective was to determine effects of equilibration length and freezing curve type as well as their interactions on motility and live spermatozoa proportion in bull sperm after thawing. The ejaculates of 6 sires were repeatedly collected. Fresh semen was diluted with one extender and divided into 3 groups equilibrated for 30, 120, and 240 min. Subsequently, half straws of each group were frozen using standard 3-phase or 2-phase freezing curve differing in the rate of temperature decrease. The spermatozoa motility (M) was evaluated immediately after thawing and at 30, 60, 90, and 120 min of thermodynamic test (TDT). Live spermatozoa proportion was evaluated after thawing and at the end of TDT. Average of spermatozoa motility (AM), decrease of spermatozoa motility (MD), average proportion of live spermatozoa (ALS), and decrease of live spermatozoa proportion (DLS) through the TDT were calculated. Significant inter-sire differences in AM (0.45-17.0%; P < 0.05-0.01), MD (0.76-12.57%; P < 0.05-0.01), and ALS (0.99-23.8%; P < 0.01) were detected. The longest equilibration ensured the highest M during TDT and AM (+2.72 and +4.58%; P < 0.05-0.01), however higher MD (+4.06%; P < 0.01) compared to standard length as well. Straws freezed using 2-phase curve achieved higher M through TDT, AM (+7.3%; P < 0.01) as well as ALS (+11.77%; P < 0.01). The 2-phase curve presented higher M compared to the 3-phase freezing curve within all equilibration lengths. Significant differences in AM, MD, and ALS (0.45-6.78%, 0.62-5.35%, and 20.79-21.11%; P < 0.05-0.01) between equilibration length vs freezing curve interactions were determined. Results document the importance of equilibration length, freezing curve, and their interaction effect on live spermatozoa proportion and sperm motility after thawing as well as necessity of individual conditions for bulls semen processing and insemination doses production.

Suggested Citation

  • M. Doležalová & L. Stádník & Z. Biniová & J. Ducháček & R. Stupka, 2016. "Equilibration and freezing interactions affecting bull sperm characteristics after thawing," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 61(11), pages 515-525.
  • Handle: RePEc:caa:jnlcjs:v:61:y:2016:i:11:id:23-2016-cjas
    DOI: 10.17221/23/2016-CJAS
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    References listed on IDEAS

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    1. J. Křišťan & A. Hatef & S.M.H. Alavi & T. Policar, 2014. "Sperm morphology, ultrastructure, and motility in pikeperch Sander lucioperca (Percidae, Teleostei) associated with various activation media," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 59(1), pages 1-10.
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    Cited by:

    1. Michala Hofmannová & Josef Přibyl & Emil Krupa & Petr Pešek, 2019. "Estimation of inbreeding effect on conception in Czech Holstein," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 64(7), pages 309-316.
    2. Zuzana Biniová & Luděk Stádník & Martina Doležalová & Jaromír Ducháček, 2018. "Effect of thawing method on bull sperm survival in ejaculates frozen in 4 ml and 8 ml volumes," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 63(10), pages 399-407.

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