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Association of novel polymorphisms in the bovine myocyte enhancer factor 2D (MEF2D) gene with carcass traits of Polish Holstein-Friesian cattle

Author

Listed:
  • E. Juszczuk-Kubiak

    (Department of Molecular Cytogenetics, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzębiec, Poland)

  • K. Wicińska

    (Department of Molecular Cytogenetics, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzębiec, Poland)

  • J. Oprządek

    (Deparment of Animal Sciences, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzębiec, Poland)

Abstract

In the present study the polymorphism of the myocyte enhancer factor 2D (MEF2D) gene was analyzed as a genetic marker candidate for carcass traits in Polish Holstein-Friesian cattle. Four novel single nucleotide polymorphisms (SNPs): HQ692911:g.93C>T in exon 8, JX088659:g.69C>T in intron 8, JX0692914:g.47C>T in intron 9 as well as JX088658:g.100G>A in the 3'flanking region were identified by DNA sequencing. By applying the PCR-RFLP method, the SNPs (g.93C>T/HaeIII and g.47C>T/Tsp45I) were genotyped in 401 Polish Holstein-Friesian bulls and their association with carcass traits was analyzed. Statistical analysis showed that the g.93C>T SNP was significantly associated with weight of lean (WLVC) and fat in valuable cuts (WFVC). Animals with the TT genotype had significantly higher WLVC (P < 0.05) and lower WFVC (P < 0.05) than those with the CC and CT genotypes. No significant relationship with carcass traits was found for the g.47C>T SNP (P > 0.05) in intron 9. Moreover, we found a significant effect of the combined genotypes onWFVC (P < 0.01), percent of lean (PLVC) (P < 0.05), and fat (PFVC) in valuable cuts (P < 0.01). This suggested that MEF2D is a strong candidate gene that affects carcass traits in cattle.

Suggested Citation

  • E. Juszczuk-Kubiak & K. Wicińska & J. Oprządek, 2013. "Association of novel polymorphisms in the bovine myocyte enhancer factor 2D (MEF2D) gene with carcass traits of Polish Holstein-Friesian cattle," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 58(6), pages 262-269.
    • Handle: RePEc:caa:jnlcjs:v:58:y:2013:i:6:id:6824-cjas
    DOI: 10.17221/6824-CJAS
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    References listed on IDEAS

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    1. Yong Zhao & Eva Samal & Deepak Srivastava, 2005. "Serum response factor regulates a muscle-specific microRNA that targets Hand2 during cardiogenesis," Nature, Nature, vol. 436(7048), pages 214-220, July.
    2. Yan Liu & Wenquan Niu & Zhijun Wu & Xiuxiu Su & Qiujin Chen & Lin Lu & Wei Jin, 2012. "Variants in Exon 11 of MEF2A Gene and Coronary Artery Disease: Evidence from a Case-Control Study, Systematic Review, and Meta-Analysis," PLOS ONE, Public Library of Science, vol. 7(2), pages 1-10, February.
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