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TaqMan allelic discrimination assay for A1 and A2 alleles of the bovine CSN2 gene

Author

Listed:
  • I. Manga

    (Agriresearch Rapotín Ltd., Rapotín, Czech Republic)

  • J. Dvořák

    (Department of Animal Morphology, Physiology and Genetics, Mendel University in Brno, Brno, Czech Republic)

Abstract

Alleles A1 and A2 of the Bos taurus CSN2 gene are the most frequent in a number of dairy cattle breeds. In this study, a new allelic discrimination assay using TaqMan fluorogenic probes was developed to detect single nucleotide substitution characterizing the A1/A2 alleles of the CSN2 gene. The method was validated using DNA samples of known genotypes with different concentrations and the results were compared with those for the commonly used problematic ACRS-PCR. We found the TaqMan method to be more effective, 100% reliable and hundred times more sensitive for testing the CSN2 genetic marker in cattle than the ACRS-PCR. As it enabled a rapid analysis of a large number of DNA samples in uniform format without previous DNA quality assessment and without the requirement for post-amplification manipulations, it presents an effective tool for the analysis of large-scale sample sets. The method was applied for testing on a sample of 120 Czech Holstein dairy cows. The observed relative genotype and allele frequencies were as follows: A1A1-0.20, A1A2-0.51, A2A2-0.29; A1-0.45, A2-0.55.

Suggested Citation

  • I. Manga & J. Dvořák, 2010. "TaqMan allelic discrimination assay for A1 and A2 alleles of the bovine CSN2 gene," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 55(8), pages 307-312.
    • Handle: RePEc:caa:jnlcjs:v:55:y:2010:i:8:id:89-2009-cjas
    DOI: 10.17221/89/2009-CJAS
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    References listed on IDEAS

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    1. J. Kučerová & A. Matějíček & O.M. Jandurová & P. Sørensen & E. Němcová & M. Štípková & T. Kott & J. Bouška & J. Frelich, 2006. "Milk protein genes CSN1S1, CSN2, CSN3, LGB and their relation to genetic values of milk production parameters in Czech Fleckvieh," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 51(6), pages 241-247.
    2. J. Čítek & L. Panicke & V. Řehout & H. Procházková, 2006. "Study of genetic distances between cattle breeds of Central Europe," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 51(10), pages 429-436.
    3. E. Hradecká & J. Čítek & L. Panicke & V. Řehout & L. Hanusová, 2008. "The relation of GH1, GHR and DGAT1 polymorphisms with estimated breeding values for milk production traits of German Holstein sires," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 53(6), pages 238-246.
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    Cited by:

    1. J. Čítek & V. Řehout & L. Hanusová & A. Míková & I. Jašková, 2011. "Polymorphisms in CGIL4, breeding value for somatic cell count and resistance to mastitis," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 56(7), pages 301-304.

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    More about this item

    Keywords

    allelic discrimination; CSN2; alleles A1 and A2; cattle;
    All these keywords.

    JEL classification:

    • A1 - General Economics and Teaching - - General Economics
    • A2 - General Economics and Teaching - - Economic Education and Teaching of Economics

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