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A novel porcine gene, USP7, differentially expressed in the musculus longissimus from Wujin and Large White pigs

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  • Liu Yongganmg

    (Key Laboratory of Animal Nutrition and Feed of Yunnan Province, Yunnan Agricultural University, Kunming, P.R. China)

  • Gao Shizheng

    (Key Laboratory of Animal Nutrition and Feed of Yunnan Province, Yunnan Agricultural University, Kunming, P.R. China)

Abstract

The mRNA differential display technique was performed to investigate differences in the gene expression in the musculus longissimus from Wujin and Large White pigs. A fragment of one differentially expressed gene was isolated and sequenced. A complete cDNA sequence was obtained using the rapid amplification of cDNA ends (RACE) method. The sequence prediction analysis revealed that the open reading frame of this gene encodes a protein of 1 103 amino acids, which is homologous with the ubiquitin specific peptidase 7 (USP7) of four species, rat (identity 98%), human (98%), mouse (98%) and chicken (95%), so it can be defined as the porcine USP7 gene. The differences in the USP7 gene expression in muscles from Wujin and Large White pigs were confirmed using semi-quantitative RT-PCR. The gene expression analysis in eight tissues of a Wujin × Large White cross showed that USP7 was expressed in all the tissues, except for fat.

Suggested Citation

  • Liu Yongganmg & Gao Shizheng, 2010. "A novel porcine gene, USP7, differentially expressed in the musculus longissimus from Wujin and Large White pigs," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 55(1), pages 37-41.
    • Handle: RePEc:caa:jnlcjs:v:55:y:2010:i:1:id:1709-cjas
    DOI: 10.17221/1709-CJAS
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    References listed on IDEAS

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    1. Muyang Li & Delin Chen & Ariel Shiloh & Jianyuan Luo & Anatoly Y. Nikolaev & Jun Qin & Wei Gu, 2002. "Deubiquitination of p53 by HAUSP is an important pathway for p53 stabilization," Nature, Nature, vol. 416(6881), pages 648-653, April.
    2. K. Bílek & A. Knoll & A. Stratil & K. Svobodová & P. Horák & R. Bechyňová & M. Van Poucke & L.J. Peelman, 2008. "Analysis of mRNA expression of CNN3, DCN, FBN2, POSTN, SPARC and YWHAQ genes in porcine foetal and adult skeletal muscles," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 53(5), pages 181-186.
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    Keywords

    pig; mRNA differential display; RACE; USP7 gene;
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