IDEAS home Printed from https://ideas.repec.org/a/caa/jnlcjs/v55y2010i1id1709-cjas.html
   My bibliography  Save this article

A novel porcine gene, USP7, differentially expressed in the musculus longissimus from Wujin and Large White pigs

Author

Listed:
  • Liu Yongganmg

    (Key Laboratory of Animal Nutrition and Feed of Yunnan Province, Yunnan Agricultural University, Kunming, P.R. China)

  • Gao Shizheng

    (Key Laboratory of Animal Nutrition and Feed of Yunnan Province, Yunnan Agricultural University, Kunming, P.R. China)

Abstract

The mRNA differential display technique was performed to investigate differences in the gene expression in the musculus longissimus from Wujin and Large White pigs. A fragment of one differentially expressed gene was isolated and sequenced. A complete cDNA sequence was obtained using the rapid amplification of cDNA ends (RACE) method. The sequence prediction analysis revealed that the open reading frame of this gene encodes a protein of 1 103 amino acids, which is homologous with the ubiquitin specific peptidase 7 (USP7) of four species, rat (identity 98%), human (98%), mouse (98%) and chicken (95%), so it can be defined as the porcine USP7 gene. The differences in the USP7 gene expression in muscles from Wujin and Large White pigs were confirmed using semi-quantitative RT-PCR. The gene expression analysis in eight tissues of a Wujin × Large White cross showed that USP7 was expressed in all the tissues, except for fat.

Suggested Citation

  • Liu Yongganmg & Gao Shizheng, 2010. "A novel porcine gene, USP7, differentially expressed in the musculus longissimus from Wujin and Large White pigs," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 55(1), pages 37-41.
    • Handle: RePEc:caa:jnlcjs:v:55:y:2010:i:1:id:1709-cjas
    DOI: 10.17221/1709-CJAS
    as

    Download full text from publisher

    File URL: http://cjas.agriculturejournals.cz/doi/10.17221/1709-CJAS.html
    Download Restriction: free of charge
    File URL: http://cjas.agriculturejournals.cz/doi/10.17221/1709-CJAS.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/1709-CJAS?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    References listed on IDEAS

    as
    1. K. Bílek & A. Knoll & A. Stratil & K. Svobodová & P. Horák & R. Bechyňová & M. Van Poucke & L.J. Peelman, 2008. "Analysis of mRNA expression of CNN3, DCN, FBN2, POSTN, SPARC and YWHAQ genes in porcine foetal and adult skeletal muscles," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 53(5), pages 181-186.
    2. Muyang Li & Delin Chen & Ariel Shiloh & Jianyuan Luo & Anatoly Y. Nikolaev & Jun Qin & Wei Gu, 2002. "Deubiquitination of p53 by HAUSP is an important pathway for p53 stabilization," Nature, Nature, vol. 416(6881), pages 648-653, April.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Hanlin Lu & Peidong Yuan & Xiaoping Ma & Xiuxin Jiang & Shaozhuang Liu & Chang Ma & Sjaak Philipsen & Qunye Zhang & Jianmin Yang & Feng Xu & Cheng Zhang & Yun Zhang & Wencheng Zhang, 2023. "Angiotensin-converting enzyme inhibitor promotes angiogenesis through Sp1/Sp3-mediated inhibition of notch signaling in male mice," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    2. Qiuhong Zhu & Panpan Liang & Hao Meng & Fangzhen Li & Wei Miao & Cuiying Chu & Wei Wang & Dongxue Li & Cong Chen & Yu Shi & Xingjiang Yu & Yifang Ping & Chaoshi Niu & Hai-bo Wu & Aili Zhang & Xiu-wu B, 2024. "Stabilization of Pin1 by USP34 promotes Ubc9 isomerization and protein sumoylation in glioma stem cells," Nature Communications, Nature, vol. 15(1), pages 1-19, December.
    3. S.M. Li & L.H. Ouyang & D.G. Zhou, 2013. "Effects of vitamin D3 on expression of defensins, Toll-like receptors, and vitamin D receptor in liver, kidney, and spleen of Silky Fowl," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 58(1), pages 1-7.

    More about this item

    Keywords

    pig; mRNA differential display; RACE; USP7 gene;
    All these keywords.

    JEL classification:

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlcjs:v:55:y:2010:i:1:id:1709-cjas. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.