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Modern methods for genetic improvement of Trifolium pratense

Author

Listed:
  • Jana ŘEPKOVÁ

    (Department of Experimental Biology, Masaryk University in Brno, Brno, Czech Republic)

  • Jan NEDĚLNÍK

    (Agricultural Research, Troubsko, Czech Republic)

Abstract

This review focuses on trends in genetic improvement of a significant representative forage crop, Trifolium pratense (red clover) classified taxonomically into the agronomically outstanding family Fabaceae. Red clover breeding is aimed at improving traits like persistency, resistance to biotic and abiotic factors, forage yield and quality characteristics such as protein quality and stability. Isoflavone content in forage is important for cattle reproduction. Interspecific hybridization of red clover with the related wild species T. medium was used for the introgression of useful traits into red clover. The breeding strategy for the new variety Pramedi included hybrid plants with different DNA contents, repeated backcrosses with red clover, followed by family selection. New techniques of molecular genetics are becoming available to breeders for transferring key and complex traits into improved red clover varieties. Techniques such as marker-assisted selection and candidate gene identification can increase the speed and precision with which traits may be selected. Comparative sequence data analyses helped to identify genes for polyphenol oxidase enzymes and isoflavone synthase in red clover useful for DNA genotyping of individuals and breeding of improved high-quality red clover varieties.

Suggested Citation

  • Jana ŘEPKOVÁ & Jan NEDĚLNÍK, 2014. "Modern methods for genetic improvement of Trifolium pratense," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 50(2), pages 92-99.
    • Handle: RePEc:caa:jnlcjg:v:50:y:2014:i:2:id:139-2013-cjgpb
    DOI: 10.17221/139/2013-CJGPB
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    References listed on IDEAS

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    1. Hana Jakešová & Jana Řepková & David Hampel & Lydie Čechová & Jan Hofbauer, 2011. "Variation of morphological and agronomic traits in hybrids of Trifolium pratense × T. medium and a comparison with the parental species," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 47(1), pages 28-36.
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