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Instrumental analytical tools for mycobacteria characterisation

Author

Listed:
  • Veronika Ozana

    (Faculty of Pharmacy, Masaryk University, Brno, Czech Republic
    Hospital with Polyclinic Karviná-Ráj Department Orlová, Czech Republic)

  • Karel Hruška

    (Veterinary Research Institute, Brno, Czech Republic
    Institute for Research and Education, Brno, Czech Republic)

Abstract

Mycobacteria in drinking water and in the water of swimming pools, whirlpools, hydrotherapy facilities and aquaria contribute significantly to human exposure to triggers of immune regulated chronic inflammatory and autoimmune diseases. Technological elements of water distribution systems, especially their inner surface, taps, shower heads and blind spots where sediments settle, affect the number of mycobacteria in the water. The review presents the possibilities of using analytical instruments for rapid determination of mycobacteria and for their typing as an alternative to classical culture and a method of monitoring specific nucleic acid sequences by polymerase chain reaction (PCR). Information about the use of flow cytometry (FCM), matrix-assisted laser desorption ionisation time-of-flight (MALDI-TOF) spectrometry, Raman and infrared (IR) spectroscopy and biosensors are presented.

Suggested Citation

  • Veronika Ozana & Karel Hruška, 2021. "Instrumental analytical tools for mycobacteria characterisation," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 39(4), pages 235-264.
    • Handle: RePEc:caa:jnlcjf:v:39:y:2021:i:4:id:69-2021-cjfs
    DOI: 10.17221/69/2021-CJFS
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    References listed on IDEAS

    as
    1. K. Hruska & M. Kaevska, 2012. "Mycobacteria in water, soil, plants and air: a review," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 57(12), pages 623-679.
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