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Bacterial biofilms resist oxidising agents due to the presence of organic matter

Author

Listed:
  • Zoran Jaglic

    (Veterinary Research Institute, Brno, Czech Republic,)

  • Dana Červinková

    (Veterinary Research Institute, Brno, Czech Republic,)

  • Hana Vlková

    (Veterinary Research Institute, Brno, Czech Republic,)

  • Elleni Michu

    (Veterinary Research Institute, Brno, Czech Republic,)

  • Gabriela Kunová

    (Dairy Research Institute, Prague, Czech Republic)

  • Vladimír Babák

    (Veterinary Research Institute, Brno, Czech Republic,)

Abstract

This study evaluated the susceptibility of planktonic and biofilm cells of Staphylococcus spp. (n = 87), Klebsiella spp. (n = 30), and Escherichia coli (n = 74) isolates originating from food contact surfaces of milk and meat processing plants to benzalkonium chloride (BAC), sodium hypochlorite (NaClO), chloramine B (CAB), and peracetic acid (PAA). Bacterial growth and reduction of viable cells in the presence of disinfectants were determined in tryptone soya broth (TSB) and water, respectively. Biofilm positive isolates (n = 73) were tested for the presence of selected qac genes. Unlike BAC, chlorine‑based disinfectants and PAA were poorly efficient in TSB, especially in the case of biofilms. However, when tested in water, the efficacy of NaClO, CAB and PAA substantially increased, which was particularly evident in biofilms. In water, staphylococcal biofilms were even more susceptible to CAB than planktonic cells. A part (23.3%) of the biofilm positive staphylococci carried the qac genes but did not express an increased resistance to BAC. This study showed that bacterial biofilms protected with organic matter could be one of the main reasons for disinfection failure.

Suggested Citation

  • Zoran Jaglic & Dana Červinková & Hana Vlková & Elleni Michu & Gabriela Kunová & Vladimír Babák, 2012. "Bacterial biofilms resist oxidising agents due to the presence of organic matter," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 30(2), pages 178-187.
    • Handle: RePEc:caa:jnlcjf:v:30:y:2012:i:2:id:214-2011-cjfs
    DOI: 10.17221/214/2011-CJFS
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    References listed on IDEAS

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    1. Jarmila Schlegelová & Vladimir Babák & Martina Holasová & Lucie Konstantinová & Lenka Necidová & Frantisek Šišák & Hana Vlková & Petr Roubal & Zoran Jaglic, 2010. "Microbial contamination after sanitation of food contact surfaces in dairy and meat processing plants," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 28(5), pages 450-461.
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    Cited by:

    1. Z. Jaglic & D. Cervinkova, 2012. "Genetic basis of resistance to quaternary ammonium compounds - the qac genes and their role: a review," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 57(6), pages 275-281.

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