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Antioxidant activity of phenolic fractions of Mallotus philippinensis bark extract

Author

Listed:
  • Muhammad ARFAN

    (Instutute of Chemical Sciences, University of Peshawar, Peshawar, Pakistan)

  • Hazrat AMIN

    (Instutute of Chemical Sciences, University of Peshawar, Peshawar, Pakistan)

  • Magdalena KARAMAĆ

    (Division of Food Science, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn, Poland)

  • Agnieszka KOSIŃSKA

    (Division of Food Science, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn, Poland)

  • Wiesław WICZKOWSKI

    (Division of Food Science, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn, Poland)

  • Ryszard AMAROWICZ

    (Division of Food Science, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn, Poland)

Abstract

Phenolic compounds were extracted from Mallotus philippinensi bark using methanol. Six fractions (I-VI) were separated from the extract on a Sephadex LH-20 column using ethanol and acetone-water as the mobile phases and were evaluated for their total antioxidant activity, antiradical activity against DPPH* (2,2-diphenyl-1-picrylhydrazyl radical), and reducing power. The total phenolics and tannin contents in the fractions were determined. The content of total phenolics in the fractions ranged from 54 mg/g (fraction I) to 927 mg/g (fraction VI). Condensed tannins were detected in fractions II-VI. Total antioxidant activity (TAA) of phenolic fractions of Mallotus philipinensis bark extract ranged from 0.58 mmol Trolox/g (fraction I) to 6.82 mmol Trolox/g (fraction IV). Fraction IV also showed the strongest antiradical activity against DPPH* and reducing power. Several phenolic constituents in the fractions were detected by RP-HPLC using a gradient solvent system with UV-DAD detection.

Suggested Citation

  • Muhammad ARFAN & Hazrat AMIN & Magdalena KARAMAĆ & Agnieszka KOSIŃSKA & Wiesław WICZKOWSKI & Ryszard AMAROWICZ, 2009. "Antioxidant activity of phenolic fractions of Mallotus philippinensis bark extract," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 27(2), pages 109-117.
    • Handle: RePEc:caa:jnlcjf:v:27:y:2009:i:2:id:1056-cjfs
    DOI: 10.17221/1056-CJFS
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    References listed on IDEAS

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    1. R. Amarowicz & S. Weidner, 2001. "Content of phenolic acids in rye caryopses determined using DAD-HPLC method," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 19(6), pages 201-205.
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