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Content of phenolic acids in rye caryopses determined using DAD-HPLC method

Author

Listed:
  • R. Amarowicz

    (Institute of Animal Reproduction and Food Research - Department of Food Science, Polish Academy of Sciences, Olsztyn)

  • S. Weidner

    (Institute of Animal Reproduction and Food Research - Department of Food Science, Polish Academy of Sciences, Olsztyn)

Abstract

Phenolic compounds were extracted from rye caryopses with 80% (v/v) methanol. Phenolic acids were determined as free compounds and those liberated from soluble esters and glycosides. The analyses were performed using a Waters HPLC system equipped with a diode array detector (DAD). The following free phenolic acids were found: p-coumaric, ferulic and sinapic; the phenolic acids liberated from soluble esters were as follows: vanillic, caffeic, p-coumaric, ferulic and sinapic; and those liberated from soluble glycosides were the following: vanillic, p-coumaric, ferulic and sinapic. In rye caryopses, phenolic acids were chiefly in the form of soluble esters. A diode array detector was especially useful for the determination of vanillic acid: the UV spectrum of this compound showed a maximum at 260 nm whereas UV spectra of other phenolic acids were characterised by maxima at longer wavelengths.

Suggested Citation

  • R. Amarowicz & S. Weidner, 2001. "Content of phenolic acids in rye caryopses determined using DAD-HPLC method," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 19(6), pages 201-205.
    • Handle: RePEc:caa:jnlcjf:v:19:y:2001:i:6:id:6608-cjfs
    DOI: 10.17221/6608-CJFS
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    Citations

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    Cited by:

    1. Muhammad ARFAN & Hazrat AMIN & Magdalena KARAMAĆ & Agnieszka KOSIŃSKA & Wiesław WICZKOWSKI & Ryszard AMAROWICZ, 2009. "Antioxidant activity of phenolic fractions of Mallotus philippinensis bark extract," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 27(2), pages 109-117.
    2. M. Karamać & R. Amarowicz & S. Weidner & S. Abe & F. Shahidi, 2002. "Antioxidant activity of rye caryopses and embryos extracts," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 20(6), pages 209-214.

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