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Antimutagenic effect of epigallocatechin gallate and its effect on the immune response in mice

Author

Listed:
  • Petr Šmerák

    (Department of General Biology and Genetics, Center of Biomedical Sciences, 3 rd Faculty of Medicine, Charles University, Prague, Czech Republic)

  • Helena Šestáková

    (National Institute of Public Health, Prague, Czech Republic)

  • Zdeňka Polívková

    (Department of General Biology and Genetics, Center of Biomedical Sciences, 3 rd Faculty of Medicine, Charles University, Prague, Czech Republic)

  • Rudolf Štětina

    (Faculty of Military Health Sciences, Department of Toxicology, Hradec Kralové, Czech Republic$2)

  • Martina Langová

    (Department of General Biology and Genetics, Center of Biomedical Sciences, 3 rd Faculty of Medicine, Charles University, Prague, Czech Republic)

  • Ivo Bárta

    (Department of General Biology and Genetics, Center of Biomedical Sciences, 3 rd Faculty of Medicine, Charles University, Prague, Czech Republic)

  • Bohumil Turek

    (National Institute of Public Health, Prague, Czech Republic)

  • Jiřina Bártová

    (Department of General Hygiene, Center of Preventive Medicine, rd 3 Faculty of Medicine, Charles University, Prague, Czech Republic)

Abstract

Green tea is the second-most consumed beverage in the world (water is the first one) and has been used medicinally for centuries in Indiaand China. The active substances in the green tea are polyphenols (catechins) and flavonols which possess a potent antioxidant activity. Epigallocatechin gallate (EGCG) is one of the four major green tea catechins. Using the Ames test, micronucleus test, comet assay, chemiluminescence test, and blastic transformation test, we examined the antimutagenic effects of chemoprotective substance epigallocatechin gallate (EGCG) in the pure form on the mutagenicity induced by three reference mutagens: aflatoxin B1 (AFB1), 2-amino-3-methylimidazo [4,5-f] qui-noline (IQ), and N-nitroso-N-methylurea (MNU), and the effect of EGCG on the immunosuppression caused by these mutagens. Using the Ames test the dose dependent antimutagenic activity of EGCG was proved against indirect mutagens AFB1 and IQ, but not against the direct mutagen MNU. In the micronucleus test, EGCG had antimutagenic effect upon all three mutagens. EGCG decreased the level of DNA breaks induced by AFB1 in bone marrow cells and colon epithelium, and the level of DNA breaks induced by MNU in colon cells to the level found in control. The reparatory effect of EGCG on immunosupression induced by all three carcinogenic compounds was proved using chemiluminescence and blastic trasformation tests.

Suggested Citation

  • Petr Šmerák & Helena Šestáková & Zdeňka Polívková & Rudolf Štětina & Martina Langová & Ivo Bárta & Bohumil Turek & Jiřina Bártová, 2006. "Antimutagenic effect of epigallocatechin gallate and its effect on the immune response in mice," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 24(4), pages 180-192.
    • Handle: RePEc:caa:jnlcjf:v:24:y:2006:i:4:id:3315-cjfs
    DOI: 10.17221/3315-CJFS
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    1. Petr Šmerák & Zdeňka Polívková & Helena Šestáková & Rudolf Štětina & Ivo Bárta & Martina Langová & Bohumil Turek & Jiřina Bártová, 2006. "Antimutagenic effect of curcumin and its effect on the immune response in mice," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 24(2), pages 72-83.
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