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Pengukuran Indole- 3-Acetic Acid (IAA) pada Bacillus sp dengan Penambahan L-Tryptopan

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  • Array Array

Abstract

Background: Asam indol asetat (IAA) merupakan salah satu hormon pertumbuhan tanaman yang berperan penting dalam menstimulasi pertumbuhan tanaman. Peran IAA yang diproduksi eksogen dari bakteri mampu mempercepat pertumbuhan tanaman dalam memacu proses diferensiasi pada akar dalam membentuk rambut akar. Bakteri rizosfer kebanyakan sebagai penghasil IAA. Bacillus sp. merupakan salah satu bakteri rizosfer dari tanaman. Tujuan penelitian ini untuk mengetahui kandungan IAA dari Bacillus sp. dengan melakukan pengukuran kadar IAA menggunakan metode kolorimetri Metode: Tahapan penelitian meliputi peremajaan isolat uji, pembuatan kurva standar IAA dan mengukur kadar IAA dengan direaksikan menggunakan reagen Salkowsky. Hasil: Hasil dari penelitian ini diperoleh kandungan IAA dari kultur Bacillus sp. yang ditambahkan L-triptofan yaitu sebesar 39,92ppm. Kesimpulan: Kandungan IAA yang dihasilkan Bacillus sp. tergolong cukup tinggi untuk dapat diaplikasikan sebagai bakteri pemacu pertumbuhan tanaman (PGPR).

Suggested Citation

  • Array Array & Array Array, 2018. "Pengukuran Indole- 3-Acetic Acid (IAA) pada Bacillus sp dengan Penambahan L-Tryptopan," BIOEDUSCIENCE, Universitas Muhammadiyah Prof. Dr. Hamka, vol. 2(2), pages 116-121.
  • Handle: RePEc:apn:bionce:v:2:y:2018:i:2:p:116-121:id:2233
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