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FTIR Study of Aqueous Solutions of α -Amylase- Evidence of C-O-H Bond Formation at High Temperatures

Author

Listed:
  • Vibhor Chaswal
  • Arshad Khan

    (Department of Chemistry, Eberly College of Science, Pennsylvania State University, USA)

Abstract

Some years ago, Lecker & Khan (Biotechnology Progress, 1996, 1998) examined the effect of heat on α-amylase by UV-VIS spectroscopy and put forward a two-step inactivation mechanism for this enzyme. In the first step Ca2+ ion dissociates reversibly from the active enzyme followed by irreversible inactivation of the apoenzyme (enzyme without the calcium attached). The purpose of this FTIR (Fourier-transform infrared spectroscopy) study is to examine what happens at the molecular level when the protein is heated from 20 to 90 °C.

Suggested Citation

  • Vibhor Chaswal & Arshad Khan, 2024. "FTIR Study of Aqueous Solutions of α -Amylase- Evidence of C-O-H Bond Formation at High Temperatures," Biomedical Journal of Scientific & Technical Research, Biomedical Research Network+, LLC, vol. 55(3), pages 47002-47007, March.
    • Handle: RePEc:abf:journl:v:55:y:2024:i:3:p:47002-47007
    DOI: 10.26717/BJSTR.2024.55.008705
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