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Preliminary Studies Towards The Production Of The Insecticide Rotenone Using Cell Culture

Author

Listed:
  • Sirju-Charran, G.
  • Maxwell, A.
  • Lackhan, N.

Abstract

Detection and quantification of rotenone in the different organs of the plant Pachyrhizus erosus (1) Urban, was achieved by High Pressure Liquid Chromatography (HPLC) using a reverse-phase column. The seeds were found to contain 0,15% rotenone on a dry weight basis, while the tubers, leaves, and the pod shells contained negligible amounts. In attempting to induce high rotenone production in vitro, leaf discs and cotyledon sections of P. erosus were used as explant and these placed on a supplement Murashige and Skoog medium. Culture, are being grown under varying condition, of light requirement, sugar source, and hormone requirement in order to determine the optimum conditions for rotenone production.

Suggested Citation

  • Sirju-Charran, G. & Maxwell, A. & Lackhan, N., 1991. "Preliminary Studies Towards The Production Of The Insecticide Rotenone Using Cell Culture," 27th Annual Meeting, July 14-20, 1991, Roseau, Dominica 259099, Caribbean Food Crops Society.
    • Handle: RePEc:ags:cfcs91:259099
    DOI: 10.22004/ag.econ.259099
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