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Bioplastics against Microplastics: Screening of Environmental Bacteria for Bioplastics Production

In: Advances and Challenges in Microplastics

Author

Listed:
  • Aisha S. Alwuhaib
  • Vitaly Zinkevich
  • Tamar Kartvelishvili
  • Nino Asatiani
  • Nelly Sapojnikova

Abstract

Polyhydroxyalkanoates (PHAs) are biopolymers produced by numerous bacteria and can be used in the production of bioplastics. PHAs are synthesized by microorganisms by fermentation of carbon sources. Due to the different monomer structures of PHAs, there are many kinds of PHAs, and their corresponding material properties are also very different. Thus, the search for bacteria producing the PHAs is of great interest. In this study, the bacteria isolated from the environment were analyzed for the presence of PHA. PHA production was tested with staining methods Sudan Black B, Nile Blue, and Nile Red. The presence of a PHA synthase gene (phaC) was confirmed by PCR amplification. PHAs were extracted from the strains and characterized by the FTIR spectroscopy method. A biochip for a fast screening of environmental samples for the presence of PHA-producing bacteria was designed. The biochip contained 11 probes for coding class 1, 2, and 3 PHA synthase genes.

Suggested Citation

  • Aisha S. Alwuhaib & Vitaly Zinkevich & Tamar Kartvelishvili & Nino Asatiani & Nelly Sapojnikova, 2023. "Bioplastics against Microplastics: Screening of Environmental Bacteria for Bioplastics Production," Chapters, in: El-Sayed Salama (ed.), Advances and Challenges in Microplastics, IntechOpen.
    • Handle: RePEc:ito:pchaps:290559
    DOI: 10.5772/intechopen.109756
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    Keywords

    polyhydroxyalkanoates (PHAs); PHA synthase gene; environmental bacteria; biochip; bioplastics;
    All these keywords.

    JEL classification:

    • Q52 - Agricultural and Natural Resource Economics; Environmental and Ecological Economics - - Environmental Economics - - - Pollution Control Adoption and Costs; Distributional Effects; Employment Effects
    • Q53 - Agricultural and Natural Resource Economics; Environmental and Ecological Economics - - Environmental Economics - - - Air Pollution; Water Pollution; Noise; Hazardous Waste; Solid Waste; Recycling

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