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Activity-Guided Isolation Of A Novel Protein From Foeniculum Vulgare With Antifungal And Antibacterial Activities

Author

Listed:
  • Hira Munir

    (Department of Biochemistry and Biotechnology, University of Gujrat, Gujrat, Pakist)

  • Muhammad Shahid

    (Protein Molecular Biology Lab, Department of Biochemistry, University of Agriculture, Faisalabad-38040, Pakistan)

  • Zinayyera Subhani

    (Protein Molecular Biology Lab, Department of Biochemistry, University of Agriculture, Faisalabad-38040, Pakistan)

  • Raheela Jabeen

    (Department of Biochemistry and Biotechnology, The Women University, Multan, Pakistan)

Abstract

This study describes the activity-guided isolation and purification of antimicrobial proteins from the seeds of Foeniculum vulgare. Purification was carried out by (NH4)2SO4 precipitation, gel filtration and ion-exchange chromatography. Antifungal and antibacterial activities were determined by disc diffusion assay method. SDS-polyacrylamide gel electrophoresis revealed that the purified protein was a monomer with molecular mass of 60 kDa. This is a first report on purification of a protein from Foeniculum vulgare, which possesses a strong and broad spectrum antimicrobial activity. Our results suggest that the antimicrobial properties of this plant seed may be further investigated to explore the possibility of using them in the treatment of candidal or bacterial infections.

Suggested Citation

  • Hira Munir & Muhammad Shahid & Zinayyera Subhani & Raheela Jabeen, 2018. "Activity-Guided Isolation Of A Novel Protein From Foeniculum Vulgare With Antifungal And Antibacterial Activities," Matrix Science Pharma (MSP), Zibeline International Publishing, vol. 2(2), pages 1-3, January.
    • Handle: RePEc:zib:zbnmsp:v:2:y:2018:i:2:p:1-3
    DOI: 10.26480/msp.02.2018.01.03
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