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COMPARISON OF THE IN VITRO CYTOTOXICITIES OF NITROGEN DOPED (p-TYPE) AND n-TYPE ZINC OXIDE NANOPARTICLES

Author

Listed:
  • JUNKO FUJIHARA

    (Department of Legal Medicine, Shimane University Faculty of Medicine, 89-1 Enya, Izumo, Shimane 693-8501, Japan)

  • HIDEKI HASHIMOTO

    (Department of Biomedical Sciences, Chubu University, 1200 Matsumoto, Kasugai, Aichi 487-8501, Japan)

  • NAOKI NISHIMOTO

    (Project for Advanced Technology Innovation, Shimane Institute for Industrial Technology, 1 Hokuryo, Matsue, Shimane 690-0816, Japan)

  • MIKI TONGU

    (Department of Pharmacy, Shin-Yamanote Hospital, Japan Anti-Tuberculosis Association, 3-6-1 Suwa, Higashimurayama, Tokyo 189-0021, Japan)

  • YASUHISA FUJITA

    (Interdisciplinary Graduate School of Science and Engineering, Shimane University, 1060 Nishikawatsu Matsue, Shimane 690-8504, Japan)

Abstract

The use of NPs in the health care field is increasing. Before their biological application, investigating the toxicities of both n-type ZnO nanoparticles (NPs) and nitrogen-doped (“p-type”) NPs is important. Using L929 cells, the cell viability, oxidative stress, apoptosis induction, inflammatory responses, and cellular uptake were assayed 24h after the addition of n-type ZnO NPs and nitrogen-doped NPs (which act as p-type) (25μg/mL). The ZnO NPs were fabricated using a gas evaporation method. Increased H2O2 generation and decreased levels of glutathione were more evident in with n-type than in those treated with nitrogen-doped (“p-type”) ZnO NPs. Caspase-3/-7 activity was higher in cells treated with n-type ZnO NPs than in those treated with nitrogen-doped (“p-type”) NPs. Elevated levels of TNF-α and IL-1β were observed in cell culture supernatants: IL-1β levels were higher in n-type ZnO NPs than nitrogen-doped (“p-type”) NPs. The cellular Zn uptake of n-type ZnO NPs was higher than nitrogen-doped (“p-type”) NPs. These findings show that n-type ZnO NPs have higher cytotoxicity than nitrogen-doped (“p-type”) ZnO NPs. This may be due to a reductive effect of n-type ZnO NPs that induces higher free radical production, reactive oxygen species (ROS) generation, and cellular uptake of this type of ZnO NPs.

Suggested Citation

  • Junko Fujihara & Hideki Hashimoto & Naoki Nishimoto & Miki Tongu & Yasuhisa Fujita, 2018. "COMPARISON OF THE IN VITRO CYTOTOXICITIES OF NITROGEN DOPED (p-TYPE) AND n-TYPE ZINC OXIDE NANOPARTICLES," Surface Review and Letters (SRL), World Scientific Publishing Co. Pte. Ltd., vol. 25(04), pages 1-8, June.
    • Handle: RePEc:wsi:srlxxx:v:25:y:2018:i:04:n:s0218625x18500841
    DOI: 10.1142/S0218625X18500841
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    Keywords

    ZnO; NPs; nitrogen-doped; n-type; toxicity; L929 cells; ROS;
    All these keywords.

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