Author
Listed:
- Somruethai Channasanon
- Pakkanun Kaewkong
- Surapol Chantaweroad
- Passakorn Tesavibul
- Yotsakorn Pratumwal
- Somboon Otarawanna
- Soshu Kirihara
- Siriporn Tanodekaew
Abstract
Geometry of porous scaffolds is critical to the success of cell adhesion, proliferation, and differentiation in bone tissue engineering. In this study, the effect of scaffold geometry on osteogenic differentiation of MC3T3-E1 pre-osteoblasts in a perfusion bioreactor was investigated. Three geometries of oligolactide-HA scaffolds, named Woodpile, LC-1000, and LC-1400, were fabricated with uniform pore size distribution and interconnectivity using stereolithography (SL) technique, and tested to evaluate for the most suitable scaffold geometry. Compressive tests revealed sufficiently high strength of all scaffolds to support new bone formation. The LC-1400 scaffold showed the highest cell proliferation in accordance with the highest level of osteoblast-specific gene expression after 21 days of dynamic culture in a perfusion bioreactor; however, it deposited less amount of calcium than the LC-1000 scaffold. Computational fluid dynamics (CFD) simulation was employed to predict and explain the effect of flow behavior on cell response under dynamic culture. The findings concluded that appropriate flow shear stress enhanced cell differentiation and mineralization in the scaffold, with the LC-1000 scaffold performing best due to its optimal balance between permeability and flow-induced shear stress.
Suggested Citation
Somruethai Channasanon & Pakkanun Kaewkong & Surapol Chantaweroad & Passakorn Tesavibul & Yotsakorn Pratumwal & Somboon Otarawanna & Soshu Kirihara & Siriporn Tanodekaew, 2024.
"Scaffold geometry and computational fluid dynamics simulation supporting osteogenic differentiation in dynamic culture,"
Computer Methods in Biomechanics and Biomedical Engineering, Taylor & Francis Journals, vol. 27(5), pages 587-598, April.
Handle:
RePEc:taf:gcmbxx:v:27:y:2024:i:5:p:587-598
DOI: 10.1080/10255842.2023.2195961
Download full text from publisher
As the access to this document is restricted, you may want to search for a different version of it.
Corrections
All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:taf:gcmbxx:v:27:y:2024:i:5:p:587-598. See general information about how to correct material in RePEc.
If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.
We have no bibliographic references for this item. You can help adding them by using this form .
If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.
For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Chris Longhurst (email available below). General contact details of provider: http://www.tandfonline.com/gcmb .
Please note that corrections may take a couple of weeks to filter through
the various RePEc services.