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Tocilizumab does not block interleukin-6 (IL-6) signaling in murine cells

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Listed:
  • Juliane Lokau
  • Florian Kleinegger
  • Yvonne Garbers
  • Georg H Waetzig
  • Joachim Grötzinger
  • Stefan Rose-John
  • Johannes Haybaeck
  • Christoph Garbers

Abstract

Tocilizumab is a humanized monoclonal antibody that is approved for the treatment of different human inflammatory diseases, including rheumatoid arthritis and cytokine release syndrome. Tocilizumab binds to the interleukin-6 receptor (IL-6R) and thereby blocks signaling of the pro-inflammatory cytokine IL-6. Initial studies and all authority assessment reports state that tocilizumab is effective in humans, but cannot bind to the murine or rat IL-6R and thus not block IL-6 signaling in the mouse. However, several recent studies described the use of tocilizumab in mice and reported biological effects that were attributed to IL-6 blockade. In this study, we investigate the capability of tocilizumab to block IL-6 signaling using different human and murine cell lines. Our results unequivocally confirm the original state of the art that tocilizumab blocks signaling via the human IL-6R, but does not block IL-6 signaling in murine cells.

Suggested Citation

  • Juliane Lokau & Florian Kleinegger & Yvonne Garbers & Georg H Waetzig & Joachim Grötzinger & Stefan Rose-John & Johannes Haybaeck & Christoph Garbers, 2020. "Tocilizumab does not block interleukin-6 (IL-6) signaling in murine cells," PLOS ONE, Public Library of Science, vol. 15(5), pages 1-10, May.
    • Handle: RePEc:plo:pone00:0232612
    DOI: 10.1371/journal.pone.0232612
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