IDEAS home Printed from https://ideas.repec.org/a/plo/pone00/0217227.html
   My bibliography  Save this article

Measure and characterization of the forces exerted by growing multicellular spheroids using microdevice arrays

Author

Listed:
  • Laurene Aoun
  • Stanislas Larnier
  • Pierre Weiss
  • Martine Cazales
  • Ariane Herbulot
  • Bernard Ducommun
  • Christophe Vieu
  • Valérie Lobjois

Abstract

Growing multicellular spheroids recapitulate many features of expanding microtumours, and therefore they are an attractive system for biomechanical studies. Here, we report an original approach to measure and characterize the forces exerted by proliferating multicellular spheroids. As force sensors, we used high aspect ratio PDMS pillars arranged as a ring that supports a growing breast tumour cell spheroid. After optical imaging and determination of the force application zones, we combined 3D reconstruction of the shape of each deformed PDMS pillar with the finite element method to extract the forces responsible for the experimental observation. We found that the force exerted by growing spheroids ranges between 100nN and 300nN. Moreover, the exerted force was dependent on the pillar stiffness and increased over time with spheroid growth.

Suggested Citation

  • Laurene Aoun & Stanislas Larnier & Pierre Weiss & Martine Cazales & Ariane Herbulot & Bernard Ducommun & Christophe Vieu & Valérie Lobjois, 2019. "Measure and characterization of the forces exerted by growing multicellular spheroids using microdevice arrays," PLOS ONE, Public Library of Science, vol. 14(5), pages 1-13, May.
    • Handle: RePEc:plo:pone00:0217227
    DOI: 10.1371/journal.pone.0217227
    as

    Download full text from publisher

    File URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0217227
    Download Restriction: no
    File URL: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0217227&type=printable
    Download Restriction: no
    File URL: https://libkey.io/10.1371/journal.pone.0217227?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. M. E. Dolega & M. Delarue & F. Ingremeau & J. Prost & A. Delon & G. Cappello, 2017. "Cell-like pressure sensors reveal increase of mechanical stress towards the core of multicellular spheroids under compression," Nature Communications, Nature, vol. 8(1), pages 1-9, April.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.

      More about this item

      Statistics

      Access and download statistics

      Corrections

      All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:plo:pone00:0217227. See general information about how to correct material in RePEc.

      If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

      If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

      If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

      For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: plosone (email available below). General contact details of provider: https://journals.plos.org/plosone/ .

      Please note that corrections may take a couple of weeks to filter through the various RePEc services.

      IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.