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Using Postmortem hippocampi tissue can interfere with differential gene expression analysis of the epileptogenic process

Author

Listed:
  • João Paulo Lopes Born
  • Heloisa de Carvalho Matos
  • Mykaella Andrade de Araujo
  • Olagide Wagner Castro
  • Marcelo Duzzioni
  • José Eduardo Peixoto-Santos
  • João Pereira Leite
  • Norberto Garcia-Cairasco
  • Maria Luisa Paçó-Larson
  • Daniel Leite Góes Gitaí

Abstract

Neuropathological studies often use autopsy brain tissue as controls to evaluate changes in protein or RNA levels in several diseases. In mesial temporal lobe epilepsy (MTLE), several genes are up or down regulated throughout the epileptogenic and chronic stages of the disease. Given that postmortem changes in several gene transcripts could impact the detection of changes in case-control studies, we evaluated the effect of using autopsy specimens with different postmortem intervals (PMI) on differential gene expression of the Pilocarpine (PILO)induced Status Epilepticus (SE) of MTLE. For this, we selected six genes (Gfap, Ppia, Gad65, Gad67, Npy, and Tnf-α) whose expression patterns in the hippocampus of PILO-injected rats are well known. Initially, we compared hippocampal expression of naïve rats whose hippocampi were harvested immediately after death (0h-PMI) with those harvested at 6h postmortem interval (6h-PMI): Npy and Ppia transcripts increased and Tnf-α transcripts decreased in the 6h-PMI group (p

Suggested Citation

  • João Paulo Lopes Born & Heloisa de Carvalho Matos & Mykaella Andrade de Araujo & Olagide Wagner Castro & Marcelo Duzzioni & José Eduardo Peixoto-Santos & João Pereira Leite & Norberto Garcia-Cairasco , 2017. "Using Postmortem hippocampi tissue can interfere with differential gene expression analysis of the epileptogenic process," PLOS ONE, Public Library of Science, vol. 12(8), pages 1-16, August.
    • Handle: RePEc:plo:pone00:0182765
    DOI: 10.1371/journal.pone.0182765
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