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Three-dimensional spatiotemporal tracking of fluorine-18 radiolabeled yeast cells via positron emission particle tracking

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  • Seth T Langford
  • Cody S Wiggins
  • Roque Santos
  • Melinda Hauser
  • Jeffrey M Becker
  • Arthur E Ruggles

Abstract

A method for Positron Emission Particle Tracking (PEPT) based on optical feature point identification techniques is demonstrated for use in low activity tracking experiments. A population of yeast cells of approximately 125,000 members is activated to roughly 55 Bq/cell by 18F uptake. An in vitro particle tracking experiment is performed with nearly 20 of these cells after decay to 32 Bq/cell. These cells are successfully identified and tracked simultaneously in this experiment. This work extends the applicability of PEPT as a cell tracking method by allowing a number of cells to be tracked together, and demonstrating tracking for very low activity tracers.

Suggested Citation

  • Seth T Langford & Cody S Wiggins & Roque Santos & Melinda Hauser & Jeffrey M Becker & Arthur E Ruggles, 2017. "Three-dimensional spatiotemporal tracking of fluorine-18 radiolabeled yeast cells via positron emission particle tracking," PLOS ONE, Public Library of Science, vol. 12(7), pages 1-14, July.
    • Handle: RePEc:plo:pone00:0180503
    DOI: 10.1371/journal.pone.0180503
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