IDEAS home Printed from https://ideas.repec.org/a/plo/pone00/0161915.html
   My bibliography  Save this article

Spheroid Formation of Hepatocarcinoma Cells in Microwells: Experiments and Monte Carlo Simulations

Author

Listed:
  • Yan Wang
  • Myung Hee Kim
  • Seyed R Tabaei
  • Jae Hyeok Park
  • Kyuhwan Na
  • Seok Chung
  • Vladimir P Zhdanov
  • Nam-Joon Cho

Abstract

The formation of spherical aggregates during the growth of cell population has long been observed under various conditions. We observed the formation of such aggregates during proliferation of Huh-7.5 cells, a human hepatocarcinoma cell line, in a microfabricated low-adhesion microwell system (SpheroFilm; formed of mass-producible silicone elastomer) on the length scales up to 500 μm. The cell proliferation was also tracked with immunofluorescence staining of F-actin and cell proliferation marker Ki-67. Meanwhile, our complementary 3D Monte Carlo simulations, taking cell diffusion and division, cell-cell and cell-scaffold adhesion, and gravity into account, illustrate the role of these factors in the formation of spheroids. Taken together, our experimental and simulation results provide an integrative view of the process of spheroid formation for Huh-7.5 cells.

Suggested Citation

  • Yan Wang & Myung Hee Kim & Seyed R Tabaei & Jae Hyeok Park & Kyuhwan Na & Seok Chung & Vladimir P Zhdanov & Nam-Joon Cho, 2016. "Spheroid Formation of Hepatocarcinoma Cells in Microwells: Experiments and Monte Carlo Simulations," PLOS ONE, Public Library of Science, vol. 11(8), pages 1-13, August.
    • Handle: RePEc:plo:pone00:0161915
    DOI: 10.1371/journal.pone.0161915
    as

    Download full text from publisher

    File URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0161915
    Download Restriction: no
    File URL: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0161915&type=printable
    Download Restriction: no
    File URL: https://libkey.io/10.1371/journal.pone.0161915?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Zhdanov, Vladimir P., 2008. "Simulation of proliferation and differentiation of cells in a stem-cell niche," Physica A: Statistical Mechanics and its Applications, Elsevier, vol. 387(24), pages 6126-6136.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Zhdanov, Vladimir P., 2009. "Signal propagation in stem-cell niches," Physica A: Statistical Mechanics and its Applications, Elsevier, vol. 388(18), pages 3791-3797.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:plo:pone00:0161915. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: plosone (email available below). General contact details of provider: https://journals.plos.org/plosone/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.