IDEAS home Printed from https://ideas.repec.org/a/plo/pone00/0157899.html
   My bibliography  Save this article

Dynamic Organization of SecA and SecY Secretion Complexes in the B. subtilis Membrane

Author

Listed:
  • Alex Dajkovic
  • Elizabeth Hinde
  • Calum MacKichan
  • Rut Carballido-Lopez

Abstract

In prokaryotes, about one third of cellular proteins are translocated across the plasma membrane or inserted into it by concerted action of the cytoplasmic ATPase SecA and the universally conserved SecYEG heterotrimeric polypeptide-translocating pore. Secretion complexes have been reported to localize in specific subcellular sites in Bacillus subtilis. In this work, we used a combination of total internal reflection microscopy, scanning fluorescence correlation spectroscopy, and pair correlation function to study the localization and dynamics of SecA and SecY in growing Bacillus subtilis cells. Both SecA and SecY localized in transient and dynamic foci in the cytoplasmic membrane, which displayed no higher-level organization in helices. Foci of SecA and SecY were in constant flux with freely diffusing SecA and SecY molecules. Scanning FCS confirmed the existence of populations of cellular SecA and SecY molecules with a wide range of diffusion coefficients. Diffusion of SecY as an uncomplexed molecular species was short-lived and only local while SecY complexed with its protein partners traversed distances of over half a micrometer in the cell.

Suggested Citation

  • Alex Dajkovic & Elizabeth Hinde & Calum MacKichan & Rut Carballido-Lopez, 2016. "Dynamic Organization of SecA and SecY Secretion Complexes in the B. subtilis Membrane," PLOS ONE, Public Library of Science, vol. 11(6), pages 1-17, June.
    • Handle: RePEc:plo:pone00:0157899
    DOI: 10.1371/journal.pone.0157899
    as

    Download full text from publisher

    File URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0157899
    Download Restriction: no
    File URL: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0157899&type=printable
    Download Restriction: no
    File URL: https://libkey.io/10.1371/journal.pone.0157899?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Tom A. Rapoport, 2007. "Protein translocation across the eukaryotic endoplasmic reticulum and bacterial plasma membranes," Nature, Nature, vol. 450(7170), pages 663-669, November.
    2. Bert van den Berg & William M. Clemons & Ian Collinson & Yorgo Modis & Enno Hartmann & Stephen C. Harrison & Tom A. Rapoport, 2004. "X-ray structure of a protein-conducting channel," Nature, Nature, vol. 427(6969), pages 36-44, January.
    3. Fredric Carlsson & Margaretha Stålhammar-Carlemalm & Klas Flärdh & Charlotta Sandin & Eric Carlemalm & Gunnar Lindahl, 2006. "Signal sequence directs localized secretion of bacterial surface proteins," Nature, Nature, vol. 442(7105), pages 943-946, August.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Xuhui Zheng & Gerben Marsman & Keenan A. Lacey & Jessica R. Chapman & Christian Goosmann & Beatrix M. Ueberheide & Victor J. Torres, 2021. "The cell envelope of Staphylococcus aureus selectively controls the sorting of virulence factors," Nature Communications, Nature, vol. 12(1), pages 1-16, December.
    2. Mohamed Mahameed & Pengli Wang & Shuai Xue & Martin Fussenegger, 2022. "Engineering receptors in the secretory pathway for orthogonal signalling control," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    3. J. Josephine Botsch & Roswitha Junker & Michèle Sorgenfrei & Patricia P. Ogger & Luca Stier & Susanne Gronau & Peter J. Murray & Markus A. Seeger & Brenda A. Schulman & Bastian Bräuning, 2024. "Doa10/MARCH6 architecture interconnects E3 ligase activity with lipid-binding transmembrane channel to regulate SQLE," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    4. Zhenrui Zhao & Mei Tian & Peng Zeng & Michael J. Christensen & Mingzhu Kou & Zhibiao Nan & Xingxu Zhang, 2022. "Modification of Cuticular Wax Composition and Biosynthesis by Epichloë gansuensis in Achnatherum inebrians at Different Growing Periods," Agriculture, MDPI, vol. 12(8), pages 1-12, August.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:plo:pone00:0157899. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: plosone (email available below). General contact details of provider: https://journals.plos.org/plosone/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.