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The Effect of Chronic Methamphetamine Exposure on the Hippocampal and Olfactory Bulb Neuroproteomes of Rats

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  • Rui Zhu
  • Tianjiao Yang
  • Firas Kobeissy
  • Tarek H Mouhieddine
  • Mohamad Raad
  • Amaly Nokkari
  • Mark S Gold
  • Kevin K Wang
  • Yehia Mechref

Abstract

Nowadays, drug abuse and addiction are serious public health problems in the USA. Methamphetamine (METH) is one of the most abused drugs and is known to cause brain damage after repeated exposure. In this paper, we conducted a neuroproteomic study to evaluate METH-induced brain protein dynamics, following a two-week chronic regimen of an escalating dose of METH exposure. Proteins were extracted from rat brain hippocampal and olfactory bulb tissues and subjected to liquid chromatography-mass spectrometry (LC-MS/MS) analysis. Both shotgun and targeted proteomic analysis were performed. Protein quantification was initially based on comparing the spectral counts between METH exposed animals and their control counterparts. Quantitative differences were further confirmed through multiple reaction monitoring (MRM) LC-MS/MS experiments. According to the quantitative results, the expression of 18 proteins (11 in the hippocampus and 7 in the olfactory bulb) underwent a significant alteration as a result of exposing rats to METH. 13 of these proteins were up-regulated after METH exposure while 5 were down-regulated. The altered proteins belonging to different structural and functional families were involved in processes such as cell death, inflammation, oxidation, and apoptosis.

Suggested Citation

  • Rui Zhu & Tianjiao Yang & Firas Kobeissy & Tarek H Mouhieddine & Mohamad Raad & Amaly Nokkari & Mark S Gold & Kevin K Wang & Yehia Mechref, 2016. "The Effect of Chronic Methamphetamine Exposure on the Hippocampal and Olfactory Bulb Neuroproteomes of Rats," PLOS ONE, Public Library of Science, vol. 11(4), pages 1-21, April.
    • Handle: RePEc:plo:pone00:0151034
    DOI: 10.1371/journal.pone.0151034
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    References listed on IDEAS

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    1. Irina N Krasnova & Zuzana Justinova & Bruce Ladenheim & Subramaniam Jayanthi & Michael T McCoy & Chanel Barnes & John E Warner & Steven R Goldberg & Jean Lud Cadet, 2010. "Methamphetamine Self-Administration Is Associated with Persistent Biochemical Alterations in Striatal and Cortical Dopaminergic Terminals in the Rat," PLOS ONE, Public Library of Science, vol. 5(1), pages 1-9, January.
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