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Quantitative DNA Analyses for Airborne Birch Pollen

Author

Listed:
  • Isabell Müller-Germann
  • Bernhard Vogel
  • Heike Vogel
  • Andreas Pauling
  • Janine Fröhlich-Nowoisky
  • Ulrich Pöschl
  • Viviane R Després

Abstract

Birch trees produce large amounts of highly allergenic pollen grains that are distributed by wind and impact human health by causing seasonal hay fever, pollen-related asthma, and other allergic diseases. Traditionally, pollen forecasts are based on conventional microscopic counting techniques that are labor-intensive and limited in the reliable identification of species. Molecular biological techniques provide an alternative approach that is less labor-intensive and enables identification of any species by its genetic fingerprint. A particularly promising method is quantitative Real-Time polymerase chain reaction (qPCR), which can be used to determine the number of DNA copies and thus pollen grains in air filter samples. During the birch pollination season in 2010 in Mainz, Germany, we collected air filter samples of fine (

Suggested Citation

  • Isabell Müller-Germann & Bernhard Vogel & Heike Vogel & Andreas Pauling & Janine Fröhlich-Nowoisky & Ulrich Pöschl & Viviane R Després, 2015. "Quantitative DNA Analyses for Airborne Birch Pollen," PLOS ONE, Public Library of Science, vol. 10(10), pages 1-17, October.
    • Handle: RePEc:plo:pone00:0140949
    DOI: 10.1371/journal.pone.0140949
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