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Proteomic Alterations in B Lymphocytes of Sensitized Mice in a Model of Chemical-Induced Asthma

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  • Steven Haenen
  • Jeroen A J Vanoirbeek
  • Vanessa De Vooght
  • Liliane Schoofs
  • Benoit Nemery
  • Elke Clynen
  • Peter H M Hoet

Abstract

Introduction and Aim: The role of B-lymphocytes in chemical-induced asthma is largely unknown. Recent work demonstrated that transferring B lymphocytes from toluene diisocyanate (TDI)-sensitized mice into naïve mice, B cell KO mice and SCID mice, triggered an asthma-like response in these mice after a subsequent TDI-challenge. We applied two-dimensional difference gel electrophoresis (2D-DIGE) to describe the “sensitized signature” of B lymphocytes comparing TDI-sensitized mice with control mice. Results: Sixteen proteins were identified that were significantly up- or down-regulated in B lymphocytes of sensitized mice. Particularly differences in the expression of cyclophilin A, cofilin 1 and zinc finger containing CCHC domain protein 11 could be correlated to the function of B lymphocytes as initiators of T lymphocyte independent asthma-like responses. Conclusion: This study revealed important alterations in the proteome of sensitized B cells in a mouse model of chemical-induced asthma, which will have an important impact on the B cell function.

Suggested Citation

  • Steven Haenen & Jeroen A J Vanoirbeek & Vanessa De Vooght & Liliane Schoofs & Benoit Nemery & Elke Clynen & Peter H M Hoet, 2015. "Proteomic Alterations in B Lymphocytes of Sensitized Mice in a Model of Chemical-Induced Asthma," PLOS ONE, Public Library of Science, vol. 10(9), pages 1-13, September.
    • Handle: RePEc:plo:pone00:0138791
    DOI: 10.1371/journal.pone.0138791
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