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Micro-CT Technique Is Well Suited for Documentation of Remodeling Processes in Murine Carotid Arteries

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  • Christoph Schürmann
  • Felix Gremse
  • Hanjoong Jo
  • Fabian Kiessling
  • Ralf P Brandes

Abstract

Background: The pathomechanisms of atherosclerosis and vascular remodelling are under intense research. Only a few in vivo tools to study these processes longitudinally in animal experiments are available. Here, we evaluated the potential of micro-CT technology. Methods: Lumen areas of the common carotid arteries (CCA) in the ApoE-/- partial carotid artery ligation mouse model were compared between in vivo and ex vivo micro-CT technique and serial histology in a total of 28 animals. AuroVist-15 nm nanoparticles were used as in vivo blood pool contrast agent in a Skyscan 1176 micro-CT at resolution of 18 μmeter voxel size and a mean x-ray dose of 0.5 Gy. For ex vivo imaging, animals were perfused with MicroFil and imaged at 9 μmeter voxel size. Lumen area was evaluated at postoperative days 7, 14, and 28 first by micro-CT followed by histology. Results: In vivo micro-CT and histology revealed lumen loss starting at day 14. The lumen profile highly correlated (r = 0.79, P

Suggested Citation

  • Christoph Schürmann & Felix Gremse & Hanjoong Jo & Fabian Kiessling & Ralf P Brandes, 2015. "Micro-CT Technique Is Well Suited for Documentation of Remodeling Processes in Murine Carotid Arteries," PLOS ONE, Public Library of Science, vol. 10(6), pages 1-11, June.
    • Handle: RePEc:plo:pone00:0130374
    DOI: 10.1371/journal.pone.0130374
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    1. Dong Ju Son & Sandeep Kumar & Wakako Takabe & Chan Woo Kim & Chih-Wen Ni & Noah Alberts-Grill & In-Hwan Jang & Sangok Kim & Wankyu Kim & Sang Won Kang & Andrew H. Baker & Jai Woong Seo & Katherine W. , 2013. "The atypical mechanosensitive microRNA-712 derived from pre-ribosomal RNA induces endothelial inflammation and atherosclerosis," Nature Communications, Nature, vol. 4(1), pages 1-15, December.
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