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Chemical and Genetic Discrimination of Cistanches Herba Based on UPLC-QTOF/MS and DNA Barcoding

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  • Sihao Zheng
  • Xue Jiang
  • Labin Wu
  • Zenghui Wang
  • Linfang Huang

Abstract

Cistanches Herba (Rou Cong Rong), known as “Ginseng of the desert”, has a striking curative effect on strength and nourishment, especially in kidney reinforcement to strengthen yang. However, the two plant origins of Cistanches Herba, Cistanche deserticola and Cistanche tubulosa, vary in terms of pharmacological action and chemical components. To discriminate the plant origin of Cistanches Herba, a combined method system of chemical and genetic –UPLC-QTOF/MS technology and DNA barcoding–were firstly employed in this study. The results indicated that three potential marker compounds (isomer of campneoside II, cistanoside C, and cistanoside A) were obtained to discriminate the two origins by PCA and OPLS-DA analyses. DNA barcoding enabled to differentiate two origins accurately. NJ tree showed that two origins clustered into two clades. Our findings demonstrate that the two origins of Cistanches Herba possess different chemical compositions and genetic variation. This is the first reported evaluation of two origins of Cistanches Herba, and the finding will facilitate quality control and its clinical application.

Suggested Citation

  • Sihao Zheng & Xue Jiang & Labin Wu & Zenghui Wang & Linfang Huang, 2014. "Chemical and Genetic Discrimination of Cistanches Herba Based on UPLC-QTOF/MS and DNA Barcoding," PLOS ONE, Public Library of Science, vol. 9(5), pages 1-11, May.
    • Handle: RePEc:plo:pone00:0098061
    DOI: 10.1371/journal.pone.0098061
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    References listed on IDEAS

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    1. Labin Wu & Xue Jiang & Linfang Huang & Shilin Chen, 2013. "Processing Technology Investigation of Loquat (Eriobotrya japonica) Leaf by Ultra-Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry Combined with Chemometrics," PLOS ONE, Public Library of Science, vol. 8(5), pages 1-9, May.
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    Cited by:

    1. Lin Li & Sihao Zheng & Josef A Brinckmann & Juan Fu & Rui Zeng & Linfang Huang & Shilin Chen, 2017. "Chemical and genetic diversity of Astragalus mongholicus grown in different eco-climatic regions," PLOS ONE, Public Library of Science, vol. 12(9), pages 1-13, September.

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