IDEAS home Printed from https://ideas.repec.org/a/plo/pone00/0075461.html
   My bibliography  Save this article

Year-Round Reproduction and Induced Spawning of Chinese Amphioxus, Branchiostoma belcheri, in Laboratory

Author

Listed:
  • Guang Li
  • ZongHuang Shu
  • Yiquan Wang

Abstract

Amphioxus is a best candidate for studying the evolutionary and developmental mechanisms of vertebrates, because of its vertebrate-like but much simpler morphology, embryonic development and genome structure. Producing live amphioxus embryos throughout the year is an ideal for comparative evolution and developmental studies. However, all amphioxus species have distinct breeding seasons in the wild and laboratory. We recently found that Chinese amphioxus B. belcheri could reproduce repeatedly beyond its natural breeding season when reared under proper conditions. In this study, we were able to extend further and produce embryos throughout the year from October 2011 to October 2012. We found all examined animals had spawned repeatedly during the examined period. In addition, both lancelets B. belcheri and B. japonicum could be induced to spawn by heat-shock method, although the induced spawning efficiency was not as high as that observed in the European lancelet. In general, we have succeeded in producing B. belcheri embryos almost daily throughout the year. This advancement will provide essential embryonic material for evolutionary and developmental studies, and have great implications for the cultivation and spawning induction of other amphioxus species.

Suggested Citation

  • Guang Li & ZongHuang Shu & Yiquan Wang, 2013. "Year-Round Reproduction and Induced Spawning of Chinese Amphioxus, Branchiostoma belcheri, in Laboratory," PLOS ONE, Public Library of Science, vol. 8(9), pages 1-1, September.
    • Handle: RePEc:plo:pone00:0075461
    DOI: 10.1371/journal.pone.0075461
    as

    Download full text from publisher

    File URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0075461
    Download Restriction: no
    File URL: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0075461&type=printable
    Download Restriction: no
    File URL: https://libkey.io/10.1371/journal.pone.0075461?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Frédéric Delsuc & Henner Brinkmann & Daniel Chourrout & Hervé Philippe, 2006. "Tunicates and not cephalochordates are the closest living relatives of vertebrates," Nature, Nature, vol. 439(7079), pages 965-968, February.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Jerry S Chen & Abygail M Gumbayan & Robert W Zeller & Joseph M Mahaffy, 2014. "An Expanded Notch-Delta Model Exhibiting Long-Range Patterning and Incorporating MicroRNA Regulation," PLOS Computational Biology, Public Library of Science, vol. 10(6), pages 1-21, June.
    2. Karma Nanglu & Rudy Lerosey-Aubril & James C. Weaver & Javier Ortega-Hernández, 2023. "A mid-Cambrian tunicate and the deep origin of the ascidiacean body plan," Nature Communications, Nature, vol. 14(1), pages 1-9, December.
    3. Lenny J. Negrón-Piñeiro & Yushi Wu & Sydney Popsuj & Diana S. José-Edwards & Alberto Stolfi & Anna Di Gregorio, 2024. "Cis-regulatory interfaces reveal the molecular mechanisms underlying the notochord gene regulatory network of Ciona," Nature Communications, Nature, vol. 15(1), pages 1-19, December.
    4. Xavier Grau-Bové & Lucie Subirana & Lydvina Meister & Anaël Soubigou & Ana Neto & Anamaria Elek & Silvia Naranjo & Oscar Fornas & Jose Luis Gomez-Skarmeta & Juan J. Tena & Manuel Irimia & Stéphanie Be, 2024. "An amphioxus neurula stage cell atlas supports a complex scenario for the emergence of vertebrate head mesoderm," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    5. R Prasobh & Narayanan Manoj, 2009. "The Repertoire of Heterotrimeric G Proteins and RGS Proteins in Ciona intestinalis," PLOS ONE, Public Library of Science, vol. 4(10), pages 1-11, October.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:plo:pone00:0075461. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: plosone (email available below). General contact details of provider: https://journals.plos.org/plosone/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.