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Pituitary Adenylate Cyclase Activating Peptide (PACAP) Participates in Adipogenesis by Activating ERK Signaling Pathway

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  • Tatjana Arsenijevic
  • Françoise Gregoire
  • Jeanne Chiadak
  • Elodie Courtequisse
  • Nargis Bolaky
  • Jason Perret
  • Christine Delporte

Abstract

Pituitary adenylate cyclase activating peptide (PACAP) belongs to the secretin/glucagon/vasoactive intestinal peptide (VIP) family. Its action can be mediated by three different receptor subtypes: PAC1, which has exclusive affinity for PACAP, and VPAC1 and VPAC2 which have equal affinity for PACAP and VIP. We showed that all three receptors are expressed in 3T3-L1 cells throughout their differentiation into adipocytes. We established the activity of these receptors by cAMP accumulation upon induction by PACAP. Together with insulin and dexamethasone, PACAP induced adipogenesis in 3T3-L1 cell line. PACAP increased cAMP production within 15 min upon stimulation and targeted the expression and phosphorylation of MAPK (ERK1/2), strengthened by the ERK1/2 phosphorylation being partially or completely abolished by different combinations of PACAP receptors antagonists. We therefore speculate that ERK1/2 activation is crucial for the activation of CCAAT/enhancer- binding protein β (C/EBPβ).

Suggested Citation

  • Tatjana Arsenijevic & Françoise Gregoire & Jeanne Chiadak & Elodie Courtequisse & Nargis Bolaky & Jason Perret & Christine Delporte, 2013. "Pituitary Adenylate Cyclase Activating Peptide (PACAP) Participates in Adipogenesis by Activating ERK Signaling Pathway," PLOS ONE, Public Library of Science, vol. 8(9), pages 1-8, September.
    • Handle: RePEc:plo:pone00:0072607
    DOI: 10.1371/journal.pone.0072607
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    1. Tatjana Arsenijevic & Françoise Grégoire & Valérie Delforge & Christine Delporte & Jason Perret, 2012. "Murine 3T3-L1 Adipocyte Cell Differentiation Model: Validated Reference Genes for qPCR Gene Expression Analysis," PLOS ONE, Public Library of Science, vol. 7(5), pages 1-8, May.
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