IDEAS home Printed from https://ideas.repec.org/a/plo/pone00/0067211.html
   My bibliography  Save this article

Optimisation of Wavelength Modulated Raman Spectroscopy: Towards High Throughput Cell Screening

Author

Listed:
  • Bavishna B Praveen
  • Michael Mazilu
  • Robert F Marchington
  • C Simon Herrington
  • Andrew Riches
  • Kishan Dholakia

Abstract

In the field of biomedicine, Raman spectroscopy is a powerful technique to discriminate between normal and cancerous cells. However the strong background signal from the sample and the instrumentation affects the efficiency of this discrimination technique. Wavelength Modulated Raman spectroscopy (WMRS) may suppress the background from the Raman spectra. In this study we demonstrate a systematic approach for optimizing the various parameters of WMRS to achieve a reduction in the acquisition time for potential applications such as higher throughput cell screening. The Signal to Noise Ratio (SNR) of the Raman bands depends on the modulation amplitude, time constant and total acquisition time. It was observed that the sampling rate does not influence the signal to noise ratio of the Raman bands if three or more wavelengths are sampled. With these optimised WMRS parameters, we increased the throughput in the binary classification of normal human urothelial cells and bladder cancer cells by reducing the total acquisition time to 6 s which is significantly lower in comparison to previous acquisition times required for the discrimination between similar cell types.

Suggested Citation

  • Bavishna B Praveen & Michael Mazilu & Robert F Marchington & C Simon Herrington & Andrew Riches & Kishan Dholakia, 2013. "Optimisation of Wavelength Modulated Raman Spectroscopy: Towards High Throughput Cell Screening," PLOS ONE, Public Library of Science, vol. 8(6), pages 1-5, June.
    • Handle: RePEc:plo:pone00:0067211
    DOI: 10.1371/journal.pone.0067211
    as

    Download full text from publisher

    File URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0067211
    Download Restriction: no
    File URL: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0067211&type=printable
    Download Restriction: no
    File URL: https://libkey.io/10.1371/journal.pone.0067211?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Derek Craig & Michael Mazilu & Kishan Dholakia, 2015. "Quantitative Detection of Pharmaceuticals Using a Combination of Paper Microfluidics and Wavelength Modulated Raman Spectroscopy," PLOS ONE, Public Library of Science, vol. 10(5), pages 1-10, May.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:plo:pone00:0067211. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: plosone (email available below). General contact details of provider: https://journals.plos.org/plosone/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.