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OpenCFU, a New Free and Open-Source Software to Count Cell Colonies and Other Circular Objects

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  • Quentin Geissmann

Abstract

Counting circular objects such as cell colonies is an important source of information for biologists. Although this task is often time-consuming and subjective, it is still predominantly performed manually. The aim of the present work is to provide a new tool to enumerate circular objects from digital pictures and video streams. Here, I demonstrate that the created program, OpenCFU, is very robust, accurate and fast. In addition, it provides control over the processing parameters and is implemented in an intuitive and modern interface. OpenCFU is a cross-platform and open-source software freely available at http://opencfu.sourceforge.net.

Suggested Citation

  • Quentin Geissmann, 2013. "OpenCFU, a New Free and Open-Source Software to Count Cell Colonies and Other Circular Objects," PLOS ONE, Public Library of Science, vol. 8(2), pages 1-10, February.
    • Handle: RePEc:plo:pone00:0054072
    DOI: 10.1371/journal.pone.0054072
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    Cited by:

    1. Aron Ferenczi & Yen Peng Chew & Erika Kroll & Charlotte Koppenfels & Andrew Hudson & Attila Molnar, 2021. "Mechanistic and genetic basis of single-strand templated repair at Cas12a-induced DNA breaks in Chlamydomonas reinhardtii," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    2. Haoqing Shen & Omer An & Xi Ren & Yangyang Song & Sze Jing Tang & Xin-Yu Ke & Jian Han & Daryl Jin Tai Tay & Vanessa Hui En Ng & Fernando Bellido Molias & Priyankaa Pitcheshwar & Ka Wai Leong & Ker-Ka, 2022. "ADARs act as potent regulators of circular transcriptome in cancer," Nature Communications, Nature, vol. 13(1), pages 1-16, December.

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