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Fluorescence Behavioral Imaging (FBI) Tracks Identity in Heterogeneous Groups of Drosophila

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  • Pavan Ramdya
  • Thomas Schaffter
  • Dario Floreano
  • Richard Benton

Abstract

Distinguishing subpopulations in group behavioral experiments can reveal the impact of differences in genetic, pharmacological and life-histories on social interactions and decision-making. Here we describe Fluorescence Behavioral Imaging (FBI), a toolkit that uses transgenic fluorescence to discriminate subpopulations, imaging hardware that simultaneously records behavior and fluorescence expression, and open-source software for automated, high-accuracy determination of genetic identity. Using FBI, we measure courtship partner choice in genetically mixed groups of Drosophila.

Suggested Citation

  • Pavan Ramdya & Thomas Schaffter & Dario Floreano & Richard Benton, 2012. "Fluorescence Behavioral Imaging (FBI) Tracks Identity in Heterogeneous Groups of Drosophila," PLOS ONE, Public Library of Science, vol. 7(11), pages 1-8, November.
    • Handle: RePEc:plo:pone00:0048381
    DOI: 10.1371/journal.pone.0048381
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    Cited by:

    1. Xi En Cheng & Zhi-Ming Qian & Shuo Hong Wang & Nan Jiang & Aike Guo & Yan Qiu Chen, 2015. "A Novel Method for Tracking Individuals of Fruit Fly Swarms Flying in a Laboratory Flight Arena," PLOS ONE, Public Library of Science, vol. 10(6), pages 1-18, June.

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