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The Leukemia-Associated Fusion Protein MN1-TEL Blocks TEL-Specific Recognition Sequences

Author

Listed:
  • W Martijn ter Haar
  • Magda A Meester-Smoor
  • Karel H M van Wely
  • Claudia C M M Schot
  • Marjolein J F W Janssen
  • Bart Geverts
  • Jacqueline Bonten
  • Gerard C Grosveld
  • Adriaan B Houtsmuller
  • Ellen C Zwarthoff

Abstract

The leukemia-associated fusion protein MN1-TEL combines the transcription-activating domains of MN1 with the DNA-binding domain of the transcriptional repressor TEL. Quantitative photobleaching experiments revealed that ∼20% of GFP-tagged MN1 and TEL is transiently immobilised, likely due to indirect or direct DNA binding, since transcription inhibition abolished immobilisation. Interestingly, ∼50% of the MN1-TEL fusion protein was immobile with much longer binding times than unfused MN1 and TEL. MN1-TEL immobilisation was not observed when the TEL DNA-binding domain was disrupted, suggesting that MN1-TEL stably occupies TEL recognition sequences, preventing binding of factors required for proper transcription regulation, which may contribute to leukemogenesis.

Suggested Citation

  • W Martijn ter Haar & Magda A Meester-Smoor & Karel H M van Wely & Claudia C M M Schot & Marjolein J F W Janssen & Bart Geverts & Jacqueline Bonten & Gerard C Grosveld & Adriaan B Houtsmuller & Ellen C, 2012. "The Leukemia-Associated Fusion Protein MN1-TEL Blocks TEL-Specific Recognition Sequences," PLOS ONE, Public Library of Science, vol. 7(9), pages 1-7, September.
    • Handle: RePEc:plo:pone00:0046085
    DOI: 10.1371/journal.pone.0046085
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