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Metabolite Profiling of a Diverse Collection of Wheat Lines Using Ultraperformance Liquid Chromatography Coupled with Time-of-Flight Mass Spectrometry

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  • Shawna B Matthews
  • Meenakshi Santra
  • Meghan M Mensack
  • Pamela Wolfe
  • Patrick F Byrne
  • Henry J Thompson

Abstract

Genetic differences among major types of wheat are well characterized; however, little is known about how these distinctions affect the small molecule profile of the wheat seed. Ethanol/water (65% v/v) extracts of seed from 45 wheat lines representing 3 genetically distinct classes, tetraploid durum (Triticum turgidum subspecies durum) (DW) and hexaploid hard and soft bread wheat (T. aestivum subspecies aestivum) (BW) were subjected to ultraperformance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC-TOF-MS). Discriminant analyses distinguished DW from BW with 100% accuracy due to differences in expression of nonpolar and polar ions, with differences attributed to sterol lipids/fatty acids and phospholipids/glycerolipids, respectively. Hard versus soft BW was distinguished with 100% accuracy by polar ions, with differences attributed to heterocyclic amines and polyketides versus phospholipid ions, respectively. This work provides a foundation for identification of metabolite profiles associated with desirable agronomic and human health traits and for assessing how environmental factors impact these characteristics.

Suggested Citation

  • Shawna B Matthews & Meenakshi Santra & Meghan M Mensack & Pamela Wolfe & Patrick F Byrne & Henry J Thompson, 2012. "Metabolite Profiling of a Diverse Collection of Wheat Lines Using Ultraperformance Liquid Chromatography Coupled with Time-of-Flight Mass Spectrometry," PLOS ONE, Public Library of Science, vol. 7(8), pages 1-14, August.
    • Handle: RePEc:plo:pone00:0044179
    DOI: 10.1371/journal.pone.0044179
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    Cited by:

    1. Laura Del Coco & Barbara Laddomada & Danilo Migoni & Giovanni Mita & Rosanna Simeone & Francesco Paolo Fanizzi, 2019. "Variability and Site Dependence of Grain Mineral Contents in Tetraploid Wheats," Sustainability, MDPI, vol. 11(3), pages 1-14, January.

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