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Clinical Utility of Insulin-Like Growth Factor 1 and 2; Determination by High Resolution Mass Spectrometry

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  • Cory Bystrom
  • Shijun Sheng
  • Ke Zhang
  • Michael Caulfield
  • Nigel J Clarke
  • Richard Reitz

Abstract

Measurement of insulin-like growth factor-1 (IGF-I) has utility for the diagnosis and management of growth disorders, but inter-assay comparison of results has been complicated by a multitude of reference standards, antibodies, detection methods, and pre-analytical preparation strategies. We developed a quantitative LC-MS method for intact IGF-I, which has advantages in throughput and complexity when compared to mass spectrometric approaches that rely on stable isotope dilution analysis of tryptic peptides. Since the method makes use of full-scan data, the assay was easily extended to provide quantitative measurement of IGF-II using the same assay protocol. The validated LC-MS assay for IGF-I and IGF-II provides accurate results across the pediatric and adult reference range and is suitable for clinical use.

Suggested Citation

  • Cory Bystrom & Shijun Sheng & Ke Zhang & Michael Caulfield & Nigel J Clarke & Richard Reitz, 2012. "Clinical Utility of Insulin-Like Growth Factor 1 and 2; Determination by High Resolution Mass Spectrometry," PLOS ONE, Public Library of Science, vol. 7(9), pages 1-7, September.
    • Handle: RePEc:plo:pone00:0043457
    DOI: 10.1371/journal.pone.0043457
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