IDEAS home Printed from https://ideas.repec.org/a/plo/pone00/0042357.html
   My bibliography  Save this article

Age-Associated Changes In Oxidative Stress and NAD+ Metabolism In Human Tissue

Author

Listed:
  • Hassina Massudi
  • Ross Grant
  • Nady Braidy
  • Jade Guest
  • Bruce Farnsworth
  • Gilles J Guillemin

Abstract

Nicotinamide adenine dinucleotide (NAD+) is an essential electron transporter in mitochondrial respiration and oxidative phosphorylation. In genomic DNA, NAD+ also represents the sole substrate for the nuclear repair enzyme, poly(ADP-ribose) polymerase (PARP) and the sirtuin family of NAD-dependent histone deacetylases. Age associated increases in oxidative nuclear damage have been associated with PARP-mediated NAD+ depletion and loss of SIRT1 activity in rodents. In this study, we further investigated whether these same associations were present in aging human tissue. Human pelvic skin samples were obtained from consenting patients aged between 15–77 and newborn babies (0–1 year old) (n = 49) previously scheduled for an unrelated surgical procedure. DNA damage correlated strongly with age in both males (p = 0.029; r = 0.490) and females (p = 0.003; r = 0.600) whereas lipid oxidation (MDA) levels increased with age in males (p = 0.004; r = 0.623) but not females (p = 0.3734; r = 0.200). PARP activity significantly increased with age in males (p

Suggested Citation

  • Hassina Massudi & Ross Grant & Nady Braidy & Jade Guest & Bruce Farnsworth & Gilles J Guillemin, 2012. "Age-Associated Changes In Oxidative Stress and NAD+ Metabolism In Human Tissue," PLOS ONE, Public Library of Science, vol. 7(7), pages 1-9, July.
    • Handle: RePEc:plo:pone00:0042357
    DOI: 10.1371/journal.pone.0042357
    as

    Download full text from publisher

    File URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0042357
    Download Restriction: no
    File URL: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0042357&type=printable
    Download Restriction: no
    File URL: https://libkey.io/10.1371/journal.pone.0042357?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Julian Mevenkamp & Yvonne M. H. Bruls & Rodrigo Mancilla & Lotte Grevendonk & Joachim E. Wildberger & Kim Brouwers & Matthijs K. C. Hesselink & Patrick Schrauwen & Joris Hoeks & Riekelt H. Houtkooper , 2024. "Development of a 31P magnetic resonance spectroscopy technique to quantify NADH and NAD+ at 3 T," Nature Communications, Nature, vol. 15(1), pages 1-13, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:plo:pone00:0042357. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: plosone (email available below). General contact details of provider: https://journals.plos.org/plosone/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.